Table 1.

Specificity of the glucagon assay

Peptide (1,000 pmol/l)Raw (450 nm)Calculated, pmol/l
Glucagon-(1-61)0.080 ± 0.010 ± 0.1
Glucagon-(19-29)0.104 ± 0.041 ± 0.2
Glicentin0.121 ± 0.073 ± 1
Oxyntomodulin0.102 ± 0.051 ± 0.3
NH2 GLP-1-(7-36)0.070 ± 0.030 ± 0.2
GLP-20.068 ± 0.060 ± 0.4
GIP-(1-30)0.062 ± 0.020 ± 0.2
NH2 PYY-(1-36)0.097 ± 0.101 ± 1
Neurotensin0.050 ± 0.060 ± 0.4
Insulin0.099 ± 0.041 ± 0.2
Mouse IgG (400 μg/ml)0.0150 ± 0.124 ± 2
Rat IgG (400 μg/ml)0.0102 ± 0.092 ± 1
  • Values are means ± SD. Cross-reactivity with various peptide hormones spiked in assay buffer at 1,000 pmol/l concentration. Absorbance and calculated levels are shown below.