Table 2.

Precision and sensitivity analysis of the glucagon ELISA

Added Amount of Glucagon, pmol/l
01251020
Assay buffer
    Measured glucagon, pmol/l0 ± 12 ± 1*3 ± 1*7 ± 2*11 ± 4*25 ± 5*
    Recovery ± SD, %NA200 ± 60*150 ± 45*140 ± 35*110 ± 45*125 ± 30*
Pooled mouse plasma
    Measured glucagon, pmol/l2 ± 22 ± 24 ± 1*8 ± 3*12 ± 2*28 ± 4*
    Recovery ± SD, %NANA100 ± 34*120 ± 40*100 ± 25*130 ± 20*
Pooled rat plasma
    Measured glucagon, pmol/l3 ± 24 ± 36 ± 2*10 ± 2*17 ± 6*31 ± 5*
    Recovery ± SD, %NA100 ± 80150 ± 40*140 ± 30*140 ± 40*140 ± 25*
  • Measured glucagon is shown as meana ± SE. Units are pmol/l, and mean recovery is shown as % ±SD of 5–7 replicated determinations of glucagon concentrations in assay buffer, charcoal-stripped pooled mouse plasma, and charcoal-stripped pooled rat plasma without and with the addition of known amounts of synthetic glucagon. Average coefficient of variation for standard curve ∼8%.

  • * Significant (P < 0.05) changes by 1-way ANOVA for repeated measurements followed by Bonferroni post hoc analysis. NA, not applicable.