Table 1.

Effects of GH and insulin on the intracellular amount of newly synthesized apoB-48 and apoB-100

GroupApoB-48 (%control)ApoB-100 (%control)Proportion of apoB-48 (%total apoB)
Control100 ± 16 100 ± 17* 67.8 ± 4.7
GH126 ± 30 56 ± 22 82.8 ± 4.3*
Insulin167 ± 45* 109 ± 26* 78.2 ± 1.3
GH + Insulin184 ± 43* 81 ± 29 84.6 ± 2.9*
  • Values are means ± SD. GH, growth hormone; apoB, apolipoprotein B. After plating, the cells were given 1 nM dexamethasone and either GH (100 ng/ml), 3 nM insulin, or a combination of GH and insulin during the last 3 days of culture. Cells were labeled for 2.5 h with a [35S]methionine-cysteine mix. Amounts of labeled apoB-48 and apoB-100 in the cells were measured as described in materials and methods. Proportion of apoB-48 (%total apoB in the cells) was calculated as apoB-48/(apoB-48 + apoB-100). Results are pooled data from 4 different experiments with 3–4 culture dishes/group. Mean value of the control group (cell cultures given 1 nM dexamethasone alone) in each experiment is given as 100%. Values with different superscripts are significantly different from each other (P < 0.05, two-way ANOVA followed by Student-Newman-Keuls test).