Table 2.

2DG uptake in Sol

2DG Uptake, μmol ⋅ g−1 ⋅ h−1Precontraction Glycogen, μmol/g wet wt
BasalSubmaximal contraction Maximal contraction
60–100 g Rats
HG1.8 ± 0.3 (10) 3.5 ± 1.0 (8)7.8 ± 1.2 (5) 53 ± 5 (23)
LG2.0 ± 0.8 (11) 4.9 ± 2.2 (6)10.2 ± 3.8 (5) 13 ± 1 (22)
200–250 g Rats
HG1.5 ± 0.1 (4) 8.6 ± 1.9 (8)23.7 ± 2.2 (12) 40 ± 4 (24)
LG1.5 ± 0.3 (5) 10 ± 2.7 (8)22.5 ± 2.2 (16) 20 ± 1 (29)
  • Values are means ± SE, with nos. of experiments indicated in parentheses. 2-Deoxy-d-glucose (2DG) uptake was measured in HG and LG Sol muscles during hindlimb perfusions at rest (Basal) and during electrical stimulation (10 min) of submaximal (100-ms trains at 2-s intervals) and maximal (200-ms trains at 1-s intervals) intensities in rats weighing 60–100 g or 200–250 g. Rats weighing 200–250 g were preconditioned and perfused exactly as the 60- to 100-g rats, except that perfusate flow was set at 20 ml/min, resulting in the same perfusion pressure and flow rate/g muscle (0.4 ml ⋅ min−1 ⋅ g muscle−1) as in smaller rats. Precontraction glycogen content was measured in soleus muscle samples obtained from basal perfusions and from nonexercised legs of contraction perfusions.