Table 3.

Proliferation characteristics of bone marrow stromal cells from intact control and HX rats under differentiation culture conditions

Assay Intact Control HX
No. of cells
Day 8 (×105) 2.1 ± 0.133.5 ± 0.23
Day 14 (×105)8.6 ± 0.24 16.3 ± 0.853-150
CFU-AP colony,day 14 21.3 ± 6.45 33.7 ± 6.33-150
CFU-f colony, day 14 34.7 ± 10.941.3 ± 7.883-150
  • Values are means ± SD of 6 replicate cultures from 4 different animals/group. CFU-alkaline phosphatase (AP) and CFU-f colony values are in numbers of colonies. Cells were allowed to attach to culture dishes for 3 days before nonadherent cells were removed and basal medium was exchanged for differentiation medium.

  • F3-150 Significant difference between age-matched intact control and HX groups as determined by Student’s t-test.