We examined the effect of early exercise training (Ex) on glucose kinetics, basal and insulin stimulated skeletal muscle (SKM) plasma membrane (PM) GLUT4 in pre- and/or postnatal nutrient restricted adult rat offspring in comparison to sedentary (Sed) state. Pre-gestational control female (Ex CON vs Sed CON) and offspring exposed to prenatal (Ex IUGR vs Sed IUGR), postnatal (Ex PNGR vs Sed PNGR), or pre- and postnatal (Ex IUGR+PNGR vs Sed IUGR+PNGR) nutrient restriction were studied. The combined effect of exercise and pre/postnatal nutrition in the Ex IUGR demonstrated positive effect on basal and glucose stimulated plasma insulin response (GSIR) with suppression of endogenous hepatic glucose production (HGP) when compared to sedentary state. Ex PNGR was hyperglycemic after glucose challenge with no change in GSIP or HGP compared to sedentary state. Ex IUGR+PNGR remained glucose tolerant with unchanged GSIP, but increased endogenous HGP compared to sedentary state. Basal SKM PM associated GLUT4 was unchanged by exercise in all four groups. While Ex PNGR and Ex IUGR+PNGR insulin-responsiveness was similar to that of Ex CON, Ex IUGR remained non-responsive to insulin. Early introduction of regular Ex in the pre-gestational female offspring had a positive effect on hepatic adaptation to GSIR and HGP in IUGR and IUGR+PNGR with no effect in PNGR. Change in insulin responsiveness of SKM GLUT4 translocation was observed in exercised IUGR+PNGR and PNGR but not in exercised IUGR.