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Am J Physiol Endocrinol Metab (July 29, 2008). doi:10.1152/ajpendo.90283.2008
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Submitted on March 10, 2008
Revised on July 15, 2008
Accepted on July 21, 2008

Markers of Glycemic Control in the Mouse: Comparisons of Six-hour and Overnight Fasted Blood Glucoses to HbA1c

Byoung-Geun Han1, Chuan-Ming Hao1, Elena E Tchekneva1, Ying-Ying Wang1, Chieh Allen Lee2, Benyamin Ebrahim, Raymond C Harris1, Timothy S Kern, David H. Wasserman3, Matthew D. Breyer4, and Zhonghua Qi1*

1 Vanderbilt University
2 Case Western Reserve University
3 Vanderbilt University School of Medicine
4 Eli Lilly and Company

* To whom correspondence should be addressed. E-mail: qi_zhonghua{at}lilly.com.

The present studies examined the relationship between fasting blood glucose and HbA1c in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of six-hour versus overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with HbA1c as determined with a handheld automated device using an immunodetection method. R2 values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with HbA1c than did blood glucose following an overnight fast. To validate the immunoassay determined HbA1c, we also measured total glycosylated hemoglobin using boronate HPLC. HbA1c values correlated well with total glycosylated hemoglobin in all three strains, but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6 hour fasting glucose provides a superior index of glycemic control and correlates more closely with HbA1c than overnight fasted blood glucose in these strains of mice.




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[Abstract] [Full Text] [PDF]


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