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INNOVATIVE METHODOLOGY

Markers of glycemic control in the mouse: comparisons of 6-h- and overnight-fasted blood glucoses to Hb A_1c

¹Department of Medicine, Vanderbilt University, Nashville, Tennessee; ²Department of Medicine, Case Western Reserve University, Cleveland, Ohio; ³Department of Molecular Physiology and Biophysics, Vanderbilt University; and ⁴The Mouse Metabolic Phenotyping Center, Vanderbilt University School of Medicine, Nashville, Tennessee

Submitted 10 March 2008 ; accepted in final form 21 July 2008

The present studies examined the relationship between fasting blood glucose and Hb A_1c in C57BL/6J, DBA/2J, and KK/HlJ mice with and without diabetes mellitus. Daily averaged blood glucose levels based on continuous glucose monitoring and effects of 6-h vs. overnight fasting on blood glucose were determined. Daily averaged blood glucose levels were highly correlated with Hb A_1c, as determined with a hand-held automated device using an immunodetection method. R² values were 0.90, 0.95, and 0.99 in KK/HIJ, C57BL/6J, and DBA/2J, respectively. Six-hour fasting blood glucose correlated more closely with the level of daily averaged blood glucose and with Hb A_1c than did blood glucose following an overnight fast. To validate the immunoassay-determined Hb A_1c, we also measured total glycosylated hemoglobin using boronate HPLC. Hb A_1c values correlated well with total glycosylated hemoglobin in all three strains but were relatively lower than total glycosylated hemoglobin in diabetic DBA/2J mice. These results show that 6-h fasting glucose provides a superior index of glycemic control and correlates more closely with Hb A_1c than overnight-fasted blood glucose in these strains of mice.

glycosylated hemoglobin; methodology; mice

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