HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 293/4/E1103    most recent 00613.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Bergman, B. C. Articles by Bessesen, D. H. Search for Related Content PubMed PubMed Citation Articles by Bergman, B. C. Articles by Bessesen, D. H.

Effects of fasting on insulin action and glucose kinetics in lean and obese men and women

University of Colorado Health Sciences Center, Aurora, Colorado

Submitted 15 November 2006 ; accepted in final form 3 August 2007

The development of insulin resistance in the obese individual could impair the ability to appropriately adjust metabolism to perturbations in energy balance. We investigated a 12- vs. 48-h fast on hepatic glucose production (R_a), peripheral glucose uptake (R_d), and skeletal muscle insulin signaling in lean and obese subjects. Healthy lean [n = 14; age = 28.0 ± 1.4 yr; body mass index (BMI) = 22.8 ± 0.42] and nondiabetic obese (n = 11; age = 34.6 ± 2.3 yr; BMI = 36.1 ± 1.5) subjects were studied following a 12- and 48-h fast during 2 h of rest and a 3-h 40 mU·m^–2·min^–1 hyperinsulinemic-euglycemic clamp (HEC). Basal glucose R_a decreased significantly from the 12- to 48-h fast (lean 1.96 ± 0.23 to 1.63 ± 0.15; obese 1.23 ± 0.07 to 1.07 ± 0.07 mg·kg^–1·min^–1; P = 0.004) and was equally suppressed during the HEC after both fasts. The increase in glucose R_d during the HEC after the 12-h fast was significantly decreased in lean and obese subjects after the 48-h fast (lean 9.03 ± 1.17 to 4.16 ± 0.34, obese 6.10 ± 0.77 to 3.56 ± 0.30 mg·kg FFM^–1·min^–1; P < 0.001). After the 12- but not the 48-h fast, insulin-stimulated AKT Ser⁴⁷³ phosphorylation was greater in lean than obese subjects. We conclude that 1) 48 h of fasting produces a marked decline in peripheral insulin action, while suppression of hepatic glucose production is maintained in lean and obese men and women; and 2) the magnitude of this decline is greater in lean vs. obese subjects.

starvation; insulin resistance; obesity

This article has been cited by other articles:

				M. R Soeters, N. M Lammers, P. F Dubbelhuis, M. Ackermans, C. F Jonkers-Schuitema, E. Fliers, H. P Sauerwein, J. M Aerts, and M. J Serlie Intermittent fasting does not affect whole-body glucose, lipid, or protein metabolism Am. J. Clinical Nutrition, November 1, 2009; 90(5): 1244 - 1251. [Abstract] [Full Text] [PDF]

				M. Harrison, D. J. O'Gorman, N. McCaffrey, M. T. Hamilton, T. W. Zderic, B. P. Carson, and N. M. Moyna Influence of acute exercise with and without carbohydrate replacement on postprandial lipid metabolism J Appl Physiol, March 1, 2009; 106(3): 943 - 949. [Abstract] [Full Text] [PDF]