HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/6/E1740    most recent 00579.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via ISI Web of Science (13) Citing Articles via Google Scholar Google Scholar Articles by Bonen, A. Articles by Luiken, J. J. F. P. Search for Related Content PubMed PubMed Citation Articles by Bonen, A. Articles by Luiken, J. J. F. P.

A null mutation in skeletal muscle FAT/CD36 reveals its essential role in insulin- and AICAR-stimulated fatty acid metabolism

¹Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada; ²Department of Molecular Genetics, Maastricht University, Maastricht, The Netherlands; ³Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Cleveland, Ohio; and ⁴Department of Biochemical Physiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands

Submitted 31 October 2006 ; accepted in final form 12 January 2007

Fatty acid translocase (FAT)/CD36 is involved in regulating the uptake of long-chain fatty acids into muscle cells. However, the contribution of FAT/CD36 to fatty acid metabolism remains unknown. We examined the role of FAT/CD36 on fatty acid metabolism in perfused muscles (soleus and red and white gastrocnemius) of wild-type (WT) and FAT/CD36 null (KO) mice. In general, in muscles of KO mice, 1) insulin sensitivity and 5-aminoimidazole-4-carboxamide-1--D-ribofuranoside (AICAR) sensitivity were normal, 2) key enzymes involved in fatty acid oxidation were altered minimally or not at all, and 3) except for an increase in soleus muscle FATP1 and FATP4, these fatty acid transporters were not altered in red and white gastrocnemius muscles, whereas plasma membrane-bound fatty acid binding protein was not altered in any muscle. In KO muscles perfused under basal conditions (i.e., no insulin, no AICAR), rates of hindquarter fatty acid oxidation were reduced by 26%. Similarly, in oxidative but not glycolytic muscles, the basal rates of triacylglycerol esterification were reduced by 40%. When muscles were perfused with insulin, the net increase in fatty acid esterification was threefold greater in the oxidative muscles of WT mice compared with the oxidative muscles in KO mice. With AICAR-stimulation, the net increase in fatty acid oxidation by hindquarter muscles was 3.7-fold greater in WT compared with KO mice. In conclusion, the present studies demonstrate that FAT/CD36 has a critical role in regulating fatty acid esterification and oxidation, particularly during stimulation with insulin or AICAR.

perfusion; palmitate; esterification; oxidation; fatty transport proteins 1 and 2; plasma membrane-bound fatty acid binding protein; 5-amino-imidazole-4-carboxamide-1--D-ribofuranoside

This article has been cited by other articles:

				L. P Turcotte and J. S Fisher Skeletal Muscle Insulin Resistance: Roles of Fatty Acid Metabolism and Exercise Physical Therapy, November 1, 2008; 88(11): 1279 - 1296. [Abstract] [Full Text] [PDF]

				K. E. Pandke, K. L. Mullen, L. A. Snook, A. Bonen, and D. J. Dyck Decreasing intramuscular phosphagen content simultaneously increases plasma membrane FAT/CD36 and GLUT4 transporter abundance Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2008; 295(3): R806 - R813. [Abstract] [Full Text] [PDF]

				C. R. Benton, G. P. Holloway, S. E. Campbell, Y. Yoshida, N. N. Tandon, J. F. C. Glatz, J. J. J. F. P. Luiken, L. L. Spriet, and A. Bonen Rosiglitazone increases fatty acid oxidation and fatty acid translocase (FAT/CD36) but not carnitine palmitoyltransferase I in rat muscle mitochondria J. Physiol., March 15, 2008; 586(6): 1755 - 1766. [Abstract] [Full Text] [PDF]

				M. Ranalletta, X. Q. Du, Y. Seki, A. S. Glenn, M. Kruse, A. Fiallo, I. Estrada, T.-S. Tsao, A. E. Stenbit, E. B. Katz, et al. Hepatic response to restoration of GLUT4 in skeletal muscle of GLUT4 null mice Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1178 - E1187. [Abstract] [Full Text] [PDF]

				C. M. Mansbach II and F. Gorelick Development and Physiological Regulation of Intestinal Lipid Absorption. II. Dietary lipid absorption, complex lipid synthesis, and the intracellular packaging and secretion of chylomicrons Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G645 - G650. [Abstract] [Full Text] [PDF]

				X.-X. Han, A. Chabowski, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen Metabolic challenges reveal impaired fatty acid metabolism and translocation of FAT/CD36 but not FABPpm in obese Zucker rat muscle Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E566 - E575. [Abstract] [Full Text] [PDF]