AJP - Endo Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 292: E1740-E1749, 2007. First published January 30, 2007; doi:10.1152/ajpendo.00579.2006
0193-1849/07 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
292/6/E1740    most recent
00579.2006v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (30)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Bonen, A.
Right arrow Articles by Luiken, J. J. F. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bonen, A.
Right arrow Articles by Luiken, J. J. F. P.

A null mutation in skeletal muscle FAT/CD36 reveals its essential role in insulin- and AICAR-stimulated fatty acid metabolism

Arend Bonen,1 Xiao-Xia Han,1 Daphna D. J. Habets,2 Maria Febbraio,3 Jan F. C. Glatz,2 and Joost J. F. P. Luiken2,4

1Department of Human Health and Nutritional Sciences, University of Guelph, Guelph, Ontario, Canada; 2Department of Molecular Genetics, Maastricht University, Maastricht, The Netherlands; 3Department of Cell Biology, Lerner Research Institute, Cleveland Clinic Cleveland, Ohio; and 4Department of Biochemical Physiology and Institute of Biomembranes, Utrecht University, Utrecht, The Netherlands

Submitted 31 October 2006 ; accepted in final form 12 January 2007

Fatty acid translocase (FAT)/CD36 is involved in regulating the uptake of long-chain fatty acids into muscle cells. However, the contribution of FAT/CD36 to fatty acid metabolism remains unknown. We examined the role of FAT/CD36 on fatty acid metabolism in perfused muscles (soleus and red and white gastrocnemius) of wild-type (WT) and FAT/CD36 null (KO) mice. In general, in muscles of KO mice, 1) insulin sensitivity and 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranoside (AICAR) sensitivity were normal, 2) key enzymes involved in fatty acid oxidation were altered minimally or not at all, and 3) except for an increase in soleus muscle FATP1 and FATP4, these fatty acid transporters were not altered in red and white gastrocnemius muscles, whereas plasma membrane-bound fatty acid binding protein was not altered in any muscle. In KO muscles perfused under basal conditions (i.e., no insulin, no AICAR), rates of hindquarter fatty acid oxidation were reduced by 26%. Similarly, in oxidative but not glycolytic muscles, the basal rates of triacylglycerol esterification were reduced by 40%. When muscles were perfused with insulin, the net increase in fatty acid esterification was threefold greater in the oxidative muscles of WT mice compared with the oxidative muscles in KO mice. With AICAR-stimulation, the net increase in fatty acid oxidation by hindquarter muscles was 3.7-fold greater in WT compared with KO mice. In conclusion, the present studies demonstrate that FAT/CD36 has a critical role in regulating fatty acid esterification and oxidation, particularly during stimulation with insulin or AICAR.

perfusion; palmitate; esterification; oxidation; fatty transport proteins 1 and 2; plasma membrane-bound fatty acid binding protein; 5-amino-imidazole-4-carboxamide-1-beta-D-ribofuranoside


Address for reprint requests and other correspondence: A. Bonen, Dept. of Human Health and Nutritional Science, Univ. of Guelph, Guelph, Ontario, Canada N1G 2W1 (e-mail: abonen{at}uoguelph.ca)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
G. P. Holloway, S. S. Jain, V. Bezaire, X. X. Han, J. F. C. Glatz, J. J. F. P. Luiken, M.-E. Harper, and A. Bonen
FAT/CD36-null mice reveal that mitochondrial FAT/CD36 is required to upregulate mitochondrial fatty acid oxidation in contracting muscle
Am J Physiol Regulatory Integrative Comp Physiol, October 1, 2009; 297(4): R960 - R967.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Fullerton, F. Hakimuddin, A. Bonen, and M. Bakovic
The Development of a Metabolic Disease Phenotype in CTP:Phosphoethanolamine Cytidylyltransferase-deficient Mice
J. Biol. Chem., September 18, 2009; 284(38): 25704 - 25713.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
J. T. McFarlan, A. Bonen, and C. G. Guglielmo
Seasonal upregulation of fatty acid transporters in flight muscles of migratory white-throated sparrows (Zonotrichia albicollis)
J. Exp. Biol., September 15, 2009; 212(18): 2934 - 2940.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
D. Sebastian, M. Guitart, C. Garcia-Martinez, C. Mauvezin, J. M. Orellana-Gavalda, D. Serra, A. M. Gomez-Foix, F. G. Hegardt, and G. Asins
Novel role of FATP1 in mitochondrial fatty acid oxidation in skeletal muscle cells
J. Lipid Res., September 1, 2009; 50(9): 1789 - 1799.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. G. Nickerson, H. Alkhateeb, C. R. Benton, J. Lally, J. Nickerson, X.-X. Han, M. H. Wilson, S. S. Jain, L. A. Snook, J. F. C. Glatz, et al.
Greater Transport Efficiencies of the Membrane Fatty Acid Transporters FAT/CD36 and FATP4 Compared with FABPpm and FATP1 and Differential Effects on Fatty Acid Esterification and Oxidation in Rat Skeletal Muscle
J. Biol. Chem., June 12, 2009; 284(24): 16522 - 16530.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. Iqbal and M. M. Hussain
Intestinal lipid absorption
Am J Physiol Endocrinol Metab, June 1, 2009; 296(6): E1183 - E1194.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
A. Bonen, X.-X. Han, N. N. Tandon, J. F. C. Glatz, J. Lally, L. A. Snook, and J. J. F. P. Luiken
FAT/CD36 expression is not ablated in spontaneously hypertensive rats
J. Lipid Res., April 1, 2009; 50(4): 740 - 748.
[Abstract] [Full Text] [PDF]

Home page
 ptjournalHome page
L. P Turcotte and J. S Fisher
Skeletal Muscle Insulin Resistance: Roles of Fatty Acid Metabolism and Exercise
Physical Therapy, November 1, 2008; 88(11): 1279 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
K. E. Pandke, K. L. Mullen, L. A. Snook, A. Bonen, and D. J. Dyck
Decreasing intramuscular phosphagen content simultaneously increases plasma membrane FAT/CD36 and GLUT4 transporter abundance
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2008; 295(3): R806 - R813.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. R. Benton, G. P. Holloway, S. E. Campbell, Y. Yoshida, N. N. Tandon, J. F. C. Glatz, J. J. J. F. P. Luiken, L. L. Spriet, and A. Bonen
Rosiglitazone increases fatty acid oxidation and fatty acid translocase (FAT/CD36) but not carnitine palmitoyltransferase I in rat muscle mitochondria
J. Physiol., March 15, 2008; 586(6): 1755 - 1766.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
M. Ranalletta, X. Q. Du, Y. Seki, A. S. Glenn, M. Kruse, A. Fiallo, I. Estrada, T.-S. Tsao, A. E. Stenbit, E. B. Katz, et al.
Hepatic response to restoration of GLUT4 in skeletal muscle of GLUT4 null mice
Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1178 - E1187.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
C. M. Mansbach II and F. Gorelick
Development and Physiological Regulation of Intestinal Lipid Absorption. II. Dietary lipid absorption, complex lipid synthesis, and the intracellular packaging and secretion of chylomicrons
Am J Physiol Gastrointest Liver Physiol, October 1, 2007; 293(4): G645 - G650.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
X.-X. Han, A. Chabowski, N. N. Tandon, J. Calles-Escandon, J. F. C. Glatz, J. J. F. P. Luiken, and A. Bonen
Metabolic challenges reveal impaired fatty acid metabolism and translocation of FAT/CD36 but not FABPpm in obese Zucker rat muscle
Am J Physiol Endocrinol Metab, August 1, 2007; 293(2): E566 - E575.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2007 by the American Physiological Society.