HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 292/4/E1018    most recent 00457.2006v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (6) Citing Articles via Google Scholar Google Scholar Articles by Navarro-Tableros, V. Articles by Hiriart, M. Search for Related Content PubMed PubMed Citation Articles by Navarro-Tableros, V. Articles by Hiriart, M.

Physiological development of insulin secretion, calcium channels, and GLUT2 expression of pancreatic rat -cells

Department of Biophysics, Instituto de Fisiología Celular, Universidad Nacional Autónoma de México, Mexico City, Mexico

Submitted 29 August 2006 ; accepted in final form 16 November 2006

Insulin secretion in mature -cells increases vigorously when extracellular glucose concentration rises. Glucose-stimulated insulin secretion depends on Ca²⁺ influx through voltage-gated Ca²⁺ channels. During fetal development, this structured response is not well established, and it is after birth that -cells acquire glucose sensitivity and a robust secretion. We compared some elements of glucose-induced insulin secretion coupling in -cells obtained from neonatal and adult rats and found that neonatal cells are functionally immature compared with adult cells. We observed that neonatal cells secrete less insulin and cannot sense changes in extracellular glucose concentrations. This could be partially explained because in neonates Ca²⁺ current density and synthesis of mRNA 1 subunit Ca²⁺ channel are lower than in adult cells. Interestingly, immunostaining for 1B, 1C, and 1D subunits in neonatal cells is similar in cytoplasm and plasma membrane, whereas it occurs predominantly in the plasma membrane in adult cells. We also observed that GLUT2 expression in adult -cells is mostly located in the membrane, whereas in neonatal cells glucose transporters are predominantly in the cytoplasm. This could explain, in part, the insensitivity to extracellular glucose in neonatal -cells. Understanding neonatal -cell physiology and maturation contributes toward a better comprehension of type 2 diabetes physiopathology, where alterations in -cells include diminished L-type Ca²⁺ channels and GLUT2 expression that results in an insufficient insulin secretion.

insulin messenger RNA; calcium currents; functional immaturity

This article has been cited by other articles:

				M. Hiriart and L. Aguilar-Bryan Channel regulation of glucose sensing in the pancreatic {beta}-cell Am J Physiol Endocrinol Metab, December 1, 2008; 295(6): E1298 - E1306. [Abstract] [Full Text] [PDF]