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This Article Full Text Full Text (PDF) All Versions of this Article: 291/5/E913    most recent 00638.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in ISI Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via ISI Web of Science (1) Citing Articles via Google Scholar Google Scholar Articles by Fernández, E. Articles by Álvarez, C. Search for Related Content PubMed PubMed Citation Articles by Fernández, E. Articles by Álvarez, C.

Undernutrition does not alter the activation of -cell neogenesis and replication in adult rats after partial pancreatectomy

¹Departamento de Bioquimica y Biologia Molecular II, Facultad de Farmacia, Universidad Complutense, Madrid, Spain; and ²Laboratory of Physiopathology of Nutrition, CNRS UMR 7059, Université Paris 7/D, Diderot, France

Submitted 19 December 2005 ; accepted in final form 28 May 2006

In previous work, we demonstrated that a 65% protein calorie food restriction started during the third trimester of gestation in rats caused a reduced -cell mass at 4 days of life that persisted until adult age. In this study with adult undernourished (U) rats, we investigated 1) whether undernutrition affects the -cell growth potential and both -cell proliferation and differentiation and 2) the implication of the IGFs, highly responsive to nutritional status, in these processes. To this end, we used the 90% pancreatectomy (Px) procedure in U and control (C) adult rats. The results show that, on day 2 after Px, -cell replication was significantly higher in C rats, whereas the -cell neogenesis was markedly increased in U/Px rats. Both the serum levels of IGF-I and the liver IGF-I mRNA expression were reduced in adult U rats before and after Px compared with C rats. Pancreatic IGF-I mRNA expression was reduced in U animals on day 0. However, on day 2 after Px, the increase of pancreatic IGF-I mRNA expression was significantly higher in U rats than in C rats. These data suggest that -cells still have the capacity to regenerate in the adult U rats, with a higher efficiency than C rats on day 2, and that both -cell neogenesis and -cell replication are stimulated. The increased pancreatic IGF-I mRNA may be instrumental in these processes.

-cell regeneration; proliferation; insulin-like growth factors