HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 291/4/E683    most recent 00252.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Boelens, P. G. Articles by Deutz, N. E. Search for Related Content PubMed PubMed Citation Articles by Boelens, P. G. Articles by Deutz, N. E.

Route of administration (enteral or parenteral) affects the contribution of L-glutamine to de novo L-arginine synthesis in mice: a stable-isotope study

¹Department of Surgery, Vrije Universiteit Medical Center, Amsterdam; and ²Department of Surgery, University of Maastricht, Maastricht, The Netherlands

Submitted 7 June 2005 ; accepted in final form 1 May 2006

A pathway from enteral L-glutamine as substrate for L-arginine synthesis is suggested by previous studies. L-Glutamine and L-glutamine dipeptides exhibit numerous beneficial effects in experimental and clinical studies. In trauma patients, enteral L-glutamine supply increased plasma L-arginine. The present study was designed to quantify the contribution of L-glutamine to the de novo L-citrulline and L-arginine synthesis in mice when L-glutamine is administered in a high dose of labeled L-glutamine or L-alanyl-L-glutamine by the enteral or parenteral route. For this purpose, male Swiss mice (n = 43) underwent a laparotomy, and catheters were inserted for sampling and infusion. A primed, constant, and continuous infusion of L-alanyl-L-[2-¹⁵N]glutamine (dipeptide groups) or L-[2-¹⁵N]glutamine (free L-glutamine groups), simultaneously with L-[ureido-¹³C,²H₂]citrulline and L-[guanidino-¹⁵N₂,²H₂]arginine, was given (steady-state model). Mice received the L-glutamine tracers intravenously (jugular vein) or enterally (duodenum). Enrichments of metabolites were measured by LC-MS. Arterial L-glutamine concentrations were the highest in the intravenous dipeptide group. L-Glutamine was converted to L-citrulline and L-arginine when L-[2-¹⁵N]glutamine and L-alanyl-L-[2-¹⁵N]glutamine were given by enteral or parenteral route. The contribution of L-glutamine to the de novo synthesis of L-citrulline and L-arginine was higher in the enteral groups when compared with the intravenous groups (P < 0.005). Therefore, the route of administration (enteral or parenteral) affects the contribution of L-glutamine, provided as free molecule or dipeptide, to the de novo synthesis of L-arginine in mice.

L-glutamine; L-arginine; enteral nutrition; parenteral nutrition; surgery

This article has been cited by other articles:

				G. C Ligthart-Melis, M. C. van de Poll, M. A. Vermeulen, P. G Boelens, M P. van den Tol, C. van Schaik, J.-P. De Bandt, N. E. Deutz, C. H. Dejong, and P. A. van Leeuwen Enteral administration of alanyl-[2-15N]glutamine contributes more to the de novo synthesis of arginine than does intravenous infusion of the dipeptide in humans Am. J. Clinical Nutrition, July 1, 2009; 90(1): 95 - 105. [Abstract] [Full Text] [PDF]

				G. C Ligthart-Melis, M. C. van de Poll, P. G Boelens, C. H. Dejong, N. E. Deutz, and P. A. van Leeuwen Glutamine is an important precursor for de novo synthesis of arginine in humans Am. J. Clinical Nutrition, May 1, 2008; 87(5): 1282 - 1289. [Abstract] [Full Text] [PDF]

				H. Xue, M. B. Sawyer, C. J. Field, L. A. Dieleman, D. Murray, and V. E. Baracos Bolus Oral Glutamine Protects Rats against CPT-11-Induced Diarrhea and Differentially Activates Cytoprotective Mechanisms in Host Intestine but Not Tumor J. Nutr., April 1, 2008; 138(4): 740 - 746. [Abstract] [Full Text] [PDF]

				J. C. Marini, A. Erez, L. Castillo, and B. Lee Interaction between murine spf-ash mutation and genetic background yields different metabolic phenotypes Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1764 - E1771. [Abstract] [Full Text] [PDF]

				G. C. Ligthart-Melis, M. C. G. van de Poll, C. H. C. Dejong, P. G. Boelens, N. E. P. Deutz, and P. A. M. van Leeuwen The Route of Administration (Enteral or Parenteral) Affects the Conversion of Isotopically Labeled L-[2-15N]Glutamine Into Citrulline and Arginine in Humans JPEN J Parenter Enteral Nutr, September 1, 2007; 31(5): 343 - 350. [Abstract] [Full Text] [PDF]