AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 290: E694-E702, 2006. First published November 1, 2005; doi:10.1152/ajpendo.00464.2005
0193-1849/06 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
290/4/E694    most recent
00464.2005v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via ISI Web of Science (12)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Wadley, G. D.
Right arrow Articles by McConell, G. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wadley, G. D.
Right arrow Articles by McConell, G. K.

Effect of exercise intensity and hypoxia on skeletal muscle AMPK signaling and substrate metabolism in humans

G. D. Wadley,1 R. S. Lee-Young,1 B. J. Canny,2 C. Wasuntarawat,3 Z. P. Chen,4 M. Hargreaves,1 B. E. Kemp,4 and G. K. McConell1

Departments of Physiology, 1The University of Melbourne, Parkville, Victoria; 2Monash University, Clayton, Victoria; 3Naresuan University, Phitsanulok, Thailand; and 4St. Vincent's Institute and Commonwealth Scientific and Industrial Research Organisation Molecular and Health Sciences, Fitzroy, Victoria, Australia

Submitted 23 September 2005 ; accepted in final form 26 October 2005

We compared in human skeletal muscle the effect of absolute vs. relative exercise intensity on AMP-activated protein kinase (AMPK) signaling and substrate metabolism under normoxic and hypoxic conditions. Eight untrained males cycled for 30 min under hypoxic conditions (11.5% O2, 111 ± 12 W, 72 ± 3% hypoxia VO2 peak; 72% Hypoxia) or under normoxic conditions (20.9% O2) matched to the same absolute (111 ± 12 W, 51 ± 1% normoxia VO2 peak; 51% Normoxia) or relative (to VO2 peak) intensity (171 ± 18 W, 73 ± 1% normoxia VO2 peak; 73% Normoxia). Increases (P < 0.05) in AMPK activity, AMPK{alpha} Thr172 phosphorylation, ACCbeta Ser221 phosphorylation, free AMP content, and glucose clearance were more influenced by the absolute than by the relative exercise intensity, being greatest in 73% Normoxia with no difference between 51% Normoxia and 72% Hypoxia. In contrast to this, increases in muscle glycogen use, muscle lactate content, and plasma catecholamine concentration were more influenced by the relative than by the absolute exercise intensity, being similar in 72% Hypoxia and 73% Normoxia, with both trials higher than in 51% Normoxia. In conclusion, increases in muscle AMPK signaling, free AMP content, and glucose disposal during exercise are largely determined by the absolute exercise intensity, whereas increases in plasma catecholamine levels, muscle glycogen use, and muscle lactate levels are more closely associated with the relative exercise intensity.

metabolic regulation; glucose kinetics; contraction


Address for reprint requests and other correspondence: G. McConell, Dept. of Physiology, Univ. of Melbourne, Parkville, 3010 Australia (e-mail: mcconell{at}unimelb.edu.au)




This article has been cited by other articles:


Home page
 J. Appl. Physiol.Home page
G. K. McConell, A. Manimmanakorn, R. S. Lee-Young, B. E. Kemp, K. C. Linden, and G. D. Wadley
Differential attenuation of AMPK activation during acute exercise following exercise training or AICAR treatment
J Appl Physiol, November 1, 2008; 105(5): 1422 - 1427.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. M. Thomson, C. A. Fick, and S. E. Gordon
AMPK activation attenuates S6K1, 4E-BP1, and eEF2 signaling responses to high-frequency electrically stimulated skeletal muscle contractions
J Appl Physiol, March 1, 2008; 104(3): 625 - 632.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
R. M. Ross, G. D. Wadley, M. G. Clark, S. Rattigan, and G. K. McConell
Local Nitric Oxide Synthase Inhibition Reduces Skeletal Muscle Glucose Uptake but Not Capillary Blood Flow During In Situ Muscle Contraction in Rats
Diabetes, December 1, 2007; 56(12): 2885 - 2892.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
T. E. Jensen, A. J. Rose, Y. Hellsten, J. F. P. Wojtaszewski, and E. A. Richter
Caffeine-induced Ca2+ release increases AMPK-dependent glucose uptake in rodent soleus muscle
Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E286 - E292.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
A. Sriwijitkamol, D. K. Coletta, E. Wajcberg, G. B. Balbontin, S. M. Reyna, J. Barrientes, P. A. Eagan, C. P. Jenkinson, E. Cersosimo, R. A. DeFronzo, et al.
Effect of Acute Exercise on AMPK Signaling in Skeletal Muscle of Subjects With Type 2 Diabetes: A Time-Course and Dose-Response Study
Diabetes, March 1, 2007; 56(3): 836 - 848.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
R. S. Lee-Young, M. J. Palmer, K. C. Linden, K. LePlastrier, B. J. Canny, M. Hargreaves, G. D. Wadley, B. E. Kemp, and G. K. McConell
Carbohydrate ingestion does not alter skeletal muscle AMPK signaling during exercise in humans
Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E566 - E573.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
C. Roepstorff, M. Thiele, T. Hillig, H. Pilegaard, E. A. Richter, J. F. P. Wojtaszewski, and B. Kiens
Higher skeletal muscle {alpha}2AMPK activation and lower energy charge and fat oxidation in men than in women during submaximal exercise
J. Physiol., July 1, 2006; 574(1): 125 - 138.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2006 by the American Physiological Society.