HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 290/3/E409    most recent 00433.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (20) Citing Articles via Google Scholar Google Scholar Articles by Welle, S. Articles by Pinkert, C. A. Search for Related Content PubMed PubMed Citation Articles by Welle, S. Articles by Pinkert, C. A.

Myofibrillar protein synthesis in myostatin-deficient mice

Departments of ¹Medicine and ²Pathology and Laboratory Medicine in the Center for Aging and Developmental Biology, University of Rochester, Rochester, New York

Submitted 9 September 2005 ; accepted in final form 3 October 2005

Either increased protein synthesis or prolonged protein half-life is necessary to support the excessive muscle growth and maintenance of enlarged muscles in myostatin-deficient mice. This issue was addressed by determining in vivo rates of myofibrillar protein synthesis in mice with constitutive myostatin deficiency (Mstn^E3/E3) or normal myostatin expression (Mstn^+/+) by measuring tracer incorporation after a systemic flooding dose of L-[ring-²H₅]phenylalanine. At 5–6 wk of age, Mstn^E3/E3 mice had increased muscle mass (40%), fractional rates of myofibrillar synthesis (14%), and protein synthesis per whole muscle (60%) relative to Mstn^+/+ mice. With maturation, fractional rates of synthesis declined >50% in parallel with decreased DNA and RNA [total, 28S rRNA, and poly(A) RNA] concentrations in muscle. At 6 mo of age, Mstn^E3/E3 mice had even greater increases in muscle mass (90%) and myofibrillar synthesis per muscle (85%) relative to Mstn^+/+ mice, but the fractional rate of synthesis was normal. Estimated myofibrillar protein half-life was not affected by myostatin deficiency. Muscle DNA concentrations were reduced in both young and mature Mstn^E3/E3 mice, whereas RNA concentrations were normal, so the ratio of RNA to DNA was 30% greater than normal in Mstn^E3/E3 mice. Thus the increased protein synthesis and RNA content per muscle in myostatin-deficient mice cannot be explained entirely by an increased number of myonuclei.

muscle growth; ribonucleic acid; deoxyribonucleic acid; ubiquitin; cathepsin B

This article has been cited by other articles:

				M. R. Morissette, S. A. Cook, C. Buranasombati, M. A. Rosenberg, and A. Rosenzweig Myostatin inhibits IGF-I-induced myotube hypertrophy through Akt Am J Physiol Cell Physiol, November 1, 2009; 297(5): 1124 - 1132. [Abstract] [Full Text] [PDF]

				S. Welle, K. Burgess, C. A. Thornton, and R. Tawil Relation between extent of myostatin depletion and muscle growth in mature mice Am J Physiol Endocrinol Metab, October 1, 2009; 297(4): E935 - E940. [Abstract] [Full Text] [PDF]

				S. Welle, A. Cardillo, M. Zanche, and R. Tawil Skeletal muscle gene expression after myostatin knockout in mature mice Physiol Genomics, August 7, 2009; 38(3): 342 - 350. [Abstract] [Full Text] [PDF]

				H. Gilson, O. Schakman, S. Kalista, P. Lause, K. Tsuchida, and J.-P. Thissen Follistatin induces muscle hypertrophy through satellite cell proliferation and inhibition of both myostatin and activin Am J Physiol Endocrinol Metab, July 1, 2009; 297(1): E157 - E164. [Abstract] [Full Text] [PDF]

				S. L. Welle Myostatin and muscle fiber size. Focus on "Smad2 and 3 transcription factors control muscle mass in adulthood" and "Myostatin reduces Akt/TORC1/p70S6K signaling, inhibiting myoblast differentiation and myotube size" Am J Physiol Cell Physiol, June 1, 2009; 296(6): C1245 - C1247. [Full Text] [PDF]

				H. Amthor, A. Otto, A. Vulin, A. Rochat, J. Dumonceaux, L. Garcia, E. Mouisel, C. Hourde, R. Macharia, M. Friedrichs, et al. Muscle hypertrophy driven by myostatin blockade does not require stem/precursor-cell activity PNAS, May 5, 2009; 106(18): 7479 - 7484. [Abstract] [Full Text] [PDF]

				M. P. Harber, J. D. Crane, J. M. Dickinson, B. Jemiolo, U. Raue, T. A. Trappe, and S. W. Trappe Protein synthesis and the expression of growth-related genes are altered by running in human vastus lateralis and soleus muscles Am J Physiol Regulatory Integrative Comp Physiol, March 1, 2009; 296(3): R708 - R714. [Abstract] [Full Text] [PDF]

				S. Welle, K. Burgess, and S. Mehta Stimulation of skeletal muscle myofibrillar protein synthesis, p70 S6 kinase phosphorylation, and ribosomal protein S6 phosphorylation by inhibition of myostatin in mature mice Am J Physiol Endocrinol Metab, March 1, 2009; 296(3): E567 - E572. [Abstract] [Full Text] [PDF]

				O Schakman, H Gilson, and J P Thissen Mechanisms of glucocorticoid-induced myopathy J. Endocrinol., April 1, 2008; 197(1): 1 - 10. [Abstract] [Full Text] [PDF]

				A. M. W. Petersen, F. Magkos, P. Atherton, A. Selby, K. Smith, M. J. Rennie, B. K. Pedersen, and B. Mittendorfer Smoking impairs muscle protein synthesis and increases the expression of myostatin and MAFbx in muscle Am J Physiol Endocrinol Metab, September 1, 2007; 293(3): E843 - E848. [Abstract] [Full Text] [PDF]

				S. Welle, K. Bhatt, C. A. Pinkert, R. Tawil, and C. A. Thornton Muscle growth after postdevelopmental myostatin gene knockout Am J Physiol Endocrinol Metab, April 1, 2007; 292(4): E985 - E991. [Abstract] [Full Text] [PDF]