AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 289: E1071-E1076, 2005. First published August 16, 2005; doi:10.1152/ajpendo.00606.2004
0193-1849/05 $8.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
289/6/E1071    most recent
00606.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (32)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Holmes, B. F.
Right arrow Articles by Dohm, G. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Holmes, B. F.
Right arrow Articles by Dohm, G. L.

Regulation of muscle GLUT4 enhancer factor and myocyte enhancer factor 2 by AMP-activated protein kinase

Burton F. Holmes,1,2 David P. Sparling,2 Ann Louise Olson,3 William W. Winder,4 and G. Lynis Dohm2

1Department of Exercise and Sport Science, Human Performance Laboratory and 2Department of Physiology, Brody School of Medicine, East Carolina University, Greenville, North Carolina; 3Department of Biochemistry, The University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma; and 4Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah

Submitted 22 December 2004 ; accepted in final form 19 May 2005

As the primary glucose transporter in skeletal muscle, GLUT4 is an important factor in the regulation of blood glucose. We previously reported that stimulation of AMP-activated protein kinase (AMPK) with 5-aminoimidazole-4-carboxamide-1-{beta}-D-ribofuranoside (AICAR) increased GLUT4 expression in muscle. GLUT4 enhancer factor (GEF) and myocyte enhancer factor 2 (MEF2) have been shown to be important for normal GLUT4 expression because deletion or truncation of the consensus sequences on the promoter causes depressed GLUT4 mRNA expression. This led to the current study to investigate possible roles for GEF and MEF2 in mediating the activation of GLUT4 gene transcription in response to AMPK. Here we show that, although AMPK does not appear to phosphorylate MEF2A, AMPK directly phosphorylates the GEF protein in vitro. MEF2 and GEF are activated in response to AMPK as we observed translocation of both to the nucleus after AICAR treatment. Nuclear MEF2 protein content was increased after 2 h, and GEF protein was increased in the nucleus 1 and 2 h post-AICAR treatment. Last, GEF and MEF2 increase in binding to the GLUT4 promoter within 2 h after AICAR treatment. Thus we conclude that GEF and MEF2 mediate the AMPK-induced increase in transcription of skeletal muscle GLUT4. AMPK can phosphorylate GEF and in response to AICAR, GEF, and MEF2 translocate to the nucleus and have increased binding to the GLUT4 promoter.

5-aminoimidazole-4-carboxamide-1-{beta}-D-ribofuranoside; glutathione S-transferase; glucose transporter 4


Address for reprint requests and other correspondence: B. F. Holmes, Dept. of Biochemistry and Molecular Physics, Univ. of Arizona, Tucson, AZ 85721 (e-mail: bfh0530{at}arizona.edu)




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. F. Ndisang, N. Lane, and A. Jadhav
Upregulation of the heme oxygenase system ameliorates postprandial and fasting hyperglycemia in type 2 diabetes
Am J Physiol Endocrinol Metab, May 1, 2009; 296(5): E1029 - E1041.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. F. Ndisang and A. Jadhav
Heme oxygenase system enhances insulin sensitivity and glucose metabolism in streptozotocin-induced diabetes
Am J Physiol Endocrinol Metab, April 1, 2009; 296(4): E829 - E841.
[Abstract] [Full Text] [PDF]

Home page
 Cardiovasc ResHome page
A. Akki and A.-M. L. Seymour
Western diet impairs metabolic remodelling and contractile efficiency in cardiac hypertrophy
Cardiovasc Res, February 15, 2009; 81(3): 610 - 617.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
G. A. Lima, G. F. Anhe, G. Giannocco, M. T. Nunes, M. L. Correa-Giannella, and U. F. Machado
Contractile activity per se induces transcriptional activation of SLC2A4 gene in soleus muscle: involvement of MEF2D, HIF-1a, and TR{alpha} transcriptional factors
Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E132 - E138.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
L. Feng, L. Gao, Q. Guan, X. Hou, Q. Wan, X. Wang, and J. Zhao
Long-term moderate ethanol consumption restores insulin sensitivity in high-fat-fed rats by increasing SLC2A4 (GLUT4) in the adipose tissue by AMP-activated protein kinase activation
J. Endocrinol., October 1, 2008; 199(1): 95 - 104.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Y. C. Long and J. R. Zierath
Influence of AMP-activated protein kinase and calcineurin on metabolic networks in skeletal muscle
Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E545 - E552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. A. H. Smith, T. A. Kohn, A. K. Chetty, and E. O. Ojuka
CaMK activation during exercise is required for histone hyperacetylation and MEF2A binding at the MEF2 site on the Glut4 gene
Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E698 - E704.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
S. L. McGee, B. J.W. van Denderen, K. F. Howlett, J. Mollica, J. D. Schertzer, B. E. Kemp, and M. Hargreaves
AMP-Activated Protein Kinase Regulates GLUT4 Transcription by Phosphorylating Histone Deacetylase 5
Diabetes, April 1, 2008; 57(4): 860 - 867.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
D. M. Thomson, S. T. Herway, N. Fillmore, H. Kim, J. D. Brown, J. R. Barrow, and W. W. Winder
AMP-activated protein kinase phosphorylates transcription factors of the CREB family
J Appl Physiol, February 1, 2008; 104(2): 429 - 438.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
S. D. Mason, H. Rundqvist, I. Papandreou, R. Duh, W. J. McNulty, R. A. Howlett, I. M. Olfert, C. J. Sundberg, N. C. Denko, L. Poellinger, et al.
HIF-1{alpha} in endurance training: suppression of oxidative metabolism
Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2059 - R2069.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. J. Krawiec, G. J. Nystrom, R. A. Frost, L. S. Jefferson, and C. H. Lang
AMP-activated protein kinase agonists increase mRNA content of the muscle-specific ubiquitin ligases MAFbx and MuRF1 in C2C12 cells
Am J Physiol Endocrinol Metab, June 1, 2007; 292(6): E1555 - E1567.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
J. A. H. Smith, M. Collins, L. A. Grobler, C. J. Magee, and E. O. Ojuka
Exercise and CaMK activation both increase the binding of MEF2A to the Glut4 promoter in skeletal muscle in vivo
Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E413 - E420.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. M. Thomson, B. B. Porter, J. H. Tall, H-J. Kim, J. R. Barrow, and W. W. Winder
Skeletal muscle and heart LKB1 deficiency causes decreased voluntary running and reduced muscle mitochondrial marker enzyme expression in mice
Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E196 - E202.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
D. G. Hardie and K. Sakamoto
AMPK: A Key Sensor of Fuel and Energy Status in Skeletal Muscle
Physiology, February 1, 2006; 21(1): 48 - 60.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2005 by the American Physiological Society.