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This Article Full Text Full Text (PDF) All Versions of this Article: 289/6/E1023    most recent 00193.2005v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (14) Citing Articles via Google Scholar Google Scholar Articles by Civitarese, A. E. Articles by Schrauwen, P. Search for Related Content PubMed PubMed Citation Articles by Civitarese, A. E. Articles by Schrauwen, P.

Glucose ingestion during exercise blunts exercise-induced gene expression of skeletal muscle fat oxidative genes

¹Pennington Biomedical Research Center, Baton Rouge, Louisiana; Departments of ²Movement Sciences and ⁴Human Biology, Nutrition and Toxicology Research Institute, Maastricht University, Maastricht, The Netherlands; and ³Romande Rehabilitation Clinic, SUVACare, Sion, Switzerland

Submitted 3 May 2005 ; accepted in final form 14 July 2005

Ingestion of carbohydrate during exercise may blunt the stimulation of fat oxidative pathways by raising plasma insulin and glucose concentrations and lowering plasma free fatty acid (FFA) levels, thereby causing a marked shift in substrate oxidation. We investigated the effects of a single 2-h bout of moderate-intensity exercise on the expression of key genes involved in fat and carbohydrate metabolism with or without glucose ingestion in seven healthy untrained men (22.7 ± 0.6 yr; body mass index: 23.8 ± 1.0 kg/m²; maximal O₂ consumption: 3.85 ± 0.21 l/min). Plasma FFA concentration increased during exercise (P < 0.01) in the fasted state but remained unchanged after glucose ingestion, whereas fat oxidation (indirect calorimetry) was higher in the fasted state vs. glucose feeding (P < 0.05). Except for a significant decrease in the expression of pyruvate dehydrogenase kinase-4 (P < 0.05), glucose ingestion during exercise produced minimal effects on the expression of genes involved in carbohydrate utilization. However, glucose ingestion resulted in a decrease in the expression of genes involved in fatty acid transport and oxidation (CD36, carnitine palmitoyltransferase-1, uncoupling protein 3, and 5'-AMP-activated protein kinase-₂; P < 0.05). In conclusion, glucose ingestion during exercise decreases the expression of genes involved in lipid metabolism rather than increasing genes involved in carbohydrate metabolism.

skeletal muscle gene expression; exercise-diet interaction

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