HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) All Versions of this Article: 288/5/E907    most recent 00551.2004v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (4) Citing Articles via Google Scholar Google Scholar Articles by Shen, W. Articles by Liechty, E. A. Search for Related Content PubMed PubMed Citation Articles by Shen, W. Articles by Liechty, E. A.

Anabolic effects of insulin and IGF-I in the ovine fetus are reduced by prolonged maternal fasting

Herman B. Wells Center for Pediatric Research, Indiana University School of Medicine, Indianapolis, Indiana

Submitted 17 November 2004 ; accepted in final form 5 January 2005

Fetal nutritional stress may result in intrauterine growth restriction and postnatal insulin resistance. To determine whether insulin resistance can begin in utero, we subjected late-gestation (130–135 days) ewes to 120 h of complete fasting and compared the results with our previous work in fed ewes (38). We determined the effect of insulin and/or recombinant human (rh)IGF-I infusion on ovine fetal phenylalanine kinetics, protein synthesis, and phenylalanine accretion. Experimental infusates were 1) saline, 2) rhIGF-I plus a replacement dose of insulin (40 nmol IGF-I/h + 16 mIU insulin/h), 3) insulin (890 mIU/h), and 4) IGF-I plus insulin (40 nmol IGF-I/h + 890 mIU insulin/h). During hormone infusion, both glucose and amino acid concentrations were clamped at basal concentrations. Amino acid infusion was required during infusion of either hormone to maintain plasma concentrations constant. However, the amount required during insulin infusion was less than during IGF-I infusion and 40% less than the amount required during identical studies in nonfasted animals. Phenylalanine used for protein synthesis and accretion was increased compared with control animals but again less so than in the nonfasted animals. In contrast to nonfasted animals, neither hormone increased the fractional synthetic rate of skeletal muscle protein nor that of plasma albumin. These results indicate that a short but severe nutritional stress can significantly alter the fetal anabolic response to insulin even when both glucose and amino acid substrate supplies are restored. Therefore, adaptive responses characterized by insulin resistance begin in utero when the fetus is subjected to sufficient nutritional stress.

protein synthesis; skeletal muscle; phenylalanine kinetics; fetal programming

This article has been cited by other articles:

				C. H. van den Akker, H. Schierbeek, T. Rietveld, A. Vermes, J. J Duvekot, E. A. Steegers, and J. B van Goudoever Human fetal albumin synthesis rates during different periods of gestation Am. J. Clinical Nutrition, October 1, 2008; 88(4): 997 - 1003. [Abstract] [Full Text] [PDF]

				L. D. Brown and W. W. Hay Jr. Effect of hyperinsulinemia on amino acid utilization and oxidation independent of glucose metabolism in the ovine fetus Am J Physiol Endocrinol Metab, December 1, 2006; 291(6): E1333 - E1340. [Abstract] [Full Text] [PDF]