AJP - Endo Watch the video to see how APS reaches out to developing nations.
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 286: E194-E200, 2004. First published October 14, 2003; doi:10.1152/ajpendo.00147.2003
0193-1849/04 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
286/2/E194    most recent
00147.2003v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (55)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Mahlapuu, M.
Right arrow Articles by Marklund, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Mahlapuu, M.
Right arrow Articles by Marklund, S.

Expression profiling of the {gamma}-subunit isoforms of AMP-activated protein kinase suggests a major role for {gamma}3 in white skeletal muscle

Margit Mahlapuu,1,* Carina Johansson,1,* Kerstin Lindgren,1 Göran Hjälm,2 Brian R. Barnes,3 Anna Krook,3 Juleen R. Zierath,3 Leif Andersson,2,4 and Stefan Marklund2

1Arexis AB, SE-413 46 Gothenburg; 2Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, and 4Department of Medical Biochemistry and Microbiology, Uppsala University, Uppsala Biomedical Center, SE-751 23 Uppsala; and 3Department of Surgical Sciences, Karolinska Institutet, SE-171 77 Stockholm, Sweden

Submitted 3 April 2003 ; accepted in final form 6 October 2003

Expression patterns of the three isoforms of the regulatory {gamma}-subunit of AMP-activated protein kinase (AMPK) were determined in various tissues from adult humans, mice, and rats, as well as in human primary muscle cells. Real-time PCR-based quantification of mRNA showed similar expression patterns in the three species and a good correlation with protein expression in mice and rats. The {gamma}3-isoform appeared highly specific to skeletal muscle, whereas {gamma}1 and {gamma}2 showed broad tissue distributions. Moreover, the proportion of white, type IIb fibers in the mouse and rat muscle samples, as indicated by real-time PCR quantification of Atp1b2 mRNA, showed a strong positive correlation with the expression of {gamma}3. In samples of white skeletal muscle, {gamma}3 clearly appeared to be the most abundant {gamma}-isoform. Differentiation of human primary muscle cells from myoblasts into multinucleated myotubes was accompanied by upregulation of {gamma}3 mRNA expression, whereas levels of {gamma}1 and {gamma}2 remained largely unchanged. However, even in these cultured myotubes, {gamma}2 was the most highly expressed isoform, indicating a considerable difference compared with adult skeletal muscle. Immunoblot analysis of mouse gastrocnemius and quadriceps muscle extracts precipitated with a {gamma}3-specific antibody showed that {gamma}3 was exclusively associated with the {alpha}2- and {beta}2-subunit isoforms. The observation that the AMPK{gamma}3 isoform is expressed primarily in white skeletal muscle, in which it is the predominant {gamma}-isoform, strongly suggests that {gamma}3 has a key role in this tissue.

adenosine monophosphate; real-time polymerase chain reaction; antibodies; mouse; rat; human


Address for reprint requests and other correspondence: S. Marklund, Dept. of Animal Breeding and Genetics, Swedish Univ. of Agricultural Sciences, Uppsala Biomedical Center, Box 597, SE-751 24 Uppsala, Sweden (E-mail: Stefan.Marklund{at}bmc.uu.se).




This article has been cited by other articles:


Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. S. Deshmukh, S. Glund, R. Z. Tom, and J. R. Zierath
Role of the AMPK{gamma}3 isoform in hypoxia-stimulated glucose transport in glycolytic skeletal muscle
Am J Physiol Endocrinol Metab, December 1, 2009; 297(6): E1388 - E1394.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
B. Mortensen, P. Poulsen, L. Wegner, K. L. Stender-Petersen, R. Ribel-Madsen, M. Friedrichsen, J. B. Birk, A. Vaag, and J. F. P. Wojtaszewski
Genetic and metabolic effects on skeletal muscle AMPK in young and older twins
Am J Physiol Endocrinol Metab, October 1, 2009; 297(4): E956 - E964.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Cell Physiol.Home page
J. T. Treebak, J. B. Birk, B. F. Hansen, G. S. Olsen, and J. F. P. Wojtaszewski
A-769662 activates AMPK {beta}1-containing complexes but induces glucose uptake through a PI3-kinase-dependent pathway in mouse skeletal muscle
Am J Physiol Cell Physiol, October 1, 2009; 297(4): C1041 - C1052.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
R. S. Lee-Young, B. J. Canny, D. E. Myers, and G. K. McConell
AMPK activation is fiber type specific in human skeletal muscle: effects of exercise and short-term exercise training
J Appl Physiol, July 1, 2009; 107(1): 283 - 289.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
G. R. Steinberg and B. E. Kemp
AMPK in Health and Disease
Physiol Rev, July 1, 2009; 89(3): 1025 - 1078.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
S. K. Park, A. M. Gunawan, T. L. Scheffler, A. L. Grant, and D. E. Gerrard
Myosin heavy chain isoform content and energy metabolism can be uncoupled in pig skeletal muscle
J Anim Sci, February 1, 2009; 87(2): 522 - 531.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. Miura, Y. Kai, Y. Kamei, C. R. Bruce, N. Kubota, M. A. Febbraio, T. Kadowaki, and O. Ezaki
{alpha}2-AMPK activity is not essential for an increase in fatty acid oxidation during low-intensity exercise
Am J Physiol Endocrinol Metab, January 1, 2009; 296(1): E47 - E55.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
E. Vieira, E. C. Nilsson, A. Nerstedt, M. Ormestad, Y. C. Long, P. M. Garcia-Roves, J. R. Zierath, and M. Mahlapuu
Relationship between AMPK and the transcriptional balance of clock-related genes in skeletal muscle
Am J Physiol Endocrinol Metab, November 1, 2008; 295(5): E1032 - E1037.
[Abstract] [Full Text] [PDF]

Home page
 J ANIM SCIHome page
V. Sibut, E. Le Bihan-Duval, S. Tesseraud, E. Godet, T. Bordeau, E. Cailleau-Audouin, P. Chartrin, M. J. Duclos, and C. Berri
Adenosine monophosphate-activated protein kinase involved in variations of muscle glycogen and breast meat quality between lean and fat chickens
J Anim Sci, November 1, 2008; 86(11): 2888 - 2896.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
Y. C. Long and J. R. Zierath
Influence of AMP-activated protein kinase and calcineurin on metabolic networks in skeletal muscle
Am J Physiol Endocrinol Metab, September 1, 2008; 295(3): E545 - E552.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Regul. Integr. Comp. Physiol.Home page
C. T. Putman, K. J. B. Martins, M. E. Gallo, G. D. Lopaschuk, J. A. Pearcey, I. M. MacLean, R. J. Saranchuk, and D. Pette
{alpha}-Catalytic subunits of 5'AMP-activated protein kinase display fiber-specific expression and are upregulated by chronic low-frequency stimulation in rat muscle
Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1325 - R1334.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
S. B. Jorgensen, J. T. Treebak, B. Viollet, P. Schjerling, S. Vaulont, J. F. P. Wojtaszewski, and E. A. Richter
Role of AMPK{alpha}2 in basal, training-, and AICAR-induced GLUT4, hexokinase II, and mitochondrial protein expression in mouse muscle
Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E331 - E339.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. Fujii, N. Jessen, and L. J. Goodyear
AMP-activated protein kinase and the regulation of glucose transport
Am J Physiol Endocrinol Metab, November 1, 2006; 291(5): E867 - E877.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
H. Yu, M. F. Hirshman, N. Fujii, J. M. Pomerleau, L. E. Peter, and L. J. Goodyear
Muscle-specific overexpression of wild type and R225Q mutant AMP-activated protein kinase {gamma}3-subunit differentially regulates glycogen accumulation
Am J Physiol Endocrinol Metab, September 1, 2006; 291(3): E557 - E565.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
J. T. Treebak, S. Glund, A. Deshmukh, D. K. Klein, Y. C. Long, T. E. Jensen, S. B. Jorgensen, B. Viollet, L. Andersson, D. Neumann, et al.
AMPK-Mediated AS160 Phosphorylation in Skeletal Muscle Is Dependent on AMPK Catalytic and Regulatory Subunits.
Diabetes, July 1, 2006; 55(7): 2051 - 2058.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. R. B. Dyck and G. D. Lopaschuk
AMPK alterations in cardiac physiology and pathology: enemy or ally?
J. Physiol., July 1, 2006; 574(1): 95 - 112.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
S. B. Jorgensen, E. A. Richter, and J. F. P. Wojtaszewski
Role of AMPK in skeletal muscle metabolic regulation and adaptation in relation to exercise
J. Physiol., July 1, 2006; 574(1): 17 - 31.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
M. Gregor, A. Zeold, S. Oehler, K. A. Marobela, P. Fuchs, G. Weigel, D. G. Hardie, and G. Wiche
Plectin scaffolds recruit energy-controlling AMP-activated protein kinase (AMPK) in differentiated myofibres
J. Cell Sci., May 1, 2006; 119(9): 1864 - 1875.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Heart Circ. Physiol.Home page
J. K. Davies, D. J. Wells, K. Liu, H. R. Whitrow, T. D. Daniel, R. Grignani, C. A. Lygate, J. E. Schneider, G. Noel, H. Watkins, et al.
Characterization of the role of {gamma}2 R531G mutation in AMP-activated protein kinase in cardiac hypertrophy and Wolff-Parkinson-White syndrome
Am J Physiol Heart Circ Physiol, May 1, 2006; 290(5): H1942 - H1951.
[Abstract] [Full Text] [PDF]

Home page
 PhysiologyHome page
D. G. Hardie and K. Sakamoto
AMPK: A Key Sensor of Fuel and Energy Status in Skeletal Muscle
Physiology, February 1, 2006; 21(1): 48 - 60.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
B. R. Barnes, Y. C. Long, T. L. Steiler, Y. Leng, D. Galuska, J. F.P. Wojtaszewski, L. Andersson, and J. R. Zierath
Changes in Exercise-Induced Gene Expression in 5'-AMP-Activated Protein Kinase {gamma}3-Null and {gamma}3 R225Q Transgenic Mice
Diabetes, December 1, 2005; 54(12): 3484 - 3489.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. W. Ryder, Y. C. Long, E. Nilsson, M. Mahlapuu, and J. R. Zierath
Effects of calcineurin activation on insulin-, AICAR- and contraction-induced glucose transport in skeletal muscle
J. Physiol., September 1, 2005; 567(2): 379 - 386.
[Abstract] [Full Text] [PDF]

Home page
 J. Appl. Physiol.Home page
N. Jessen and L. J. Goodyear
Contraction signaling to glucose transport in skeletal muscle
J Appl Physiol, July 1, 2005; 99(1): 330 - 337.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
D. C. Wright, P. C. Geiger, J. O. Holloszy, and D.-H. Han
Contraction- and hypoxia-stimulated glucose transport is mediated by a Ca2+-dependent mechanism in slow-twitch rat soleus muscle
Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1062 - E1066.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
J. F. P Wojtaszewski, J. B Birk, C. Frosig, M. Holten, H. Pilegaard, and F. Dela
5'AMP activated protein kinase expression in human skeletal muscle: effects of strength training and type 2 diabetes
J. Physiol., April 15, 2005; 564(2): 563 - 573.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Renal Physiol.Home page
S. Fraser, P. Mount, R. Hill, V. Levidiotis, F. Katsis, D. Stapleton, B. E. Kemp, and D. A. Power
Regulation of the energy sensor AMP-activated protein kinase in the kidney by dietary salt intake and osmolality
Am J Physiol Renal Physiol, March 1, 2005; 288(3): F578 - F586.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. R. Barnes, S. Marklund, T. L. Steiler, M. Walter, G. Hjalm,, V. Amarger, M. Mahlapuu, Y. Leng, C. Johansson, D. Galuska, et al.
The 5'-AMP-activated Protein Kinase {gamma}3 Isoform Has a Key Role in Carbohydrate and Lipid Metabolism in Glycolytic Skeletal Muscle
J. Biol. Chem., September 10, 2004; 279(37): 38441 - 38447.
[Abstract] [Full Text] [PDF]

Home page
 DiabetesHome page
M. A. Iglesias, S. M. Furler, G. J. Cooney, E. W. Kraegen, and J.-M. Ye
AMP-Activated Protein Kinase Activation by AICAR Increases Both Muscle Fatty Acid and Glucose Uptake in White Muscle of Insulin-Resistant Rats In Vivo
Diabetes, July 1, 2004; 53(7): 1649 - 1654.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.