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This Article Full Text Full Text (PDF) All Versions of this Article: 285/3/E498    most recent 00121.2003v1 Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Web of Science (5) Citing Articles via Google Scholar Google Scholar Articles by Kowluru, A. Search for Related Content PubMed PubMed Citation Articles by Kowluru, A.

Defective protein histidine phosphorylation in islets from the Goto-Kakizaki diabetic rat

Department of Pharmaceutical Sciences, Wayne State University, Detroit 48202, and -Cell Biochemistry Research Laboratory, John D. Dingell Veterans Affairs Medical Center, Detroit, Michigan 48201

Submitted 21 March 2003 ; accepted in final form 30 April 2003

We recently described novel regulatory roles for protein histidine phosphorylation of key islet proteins (e.g., nucleoside diphosphate kinase and succinyl thiokinase) in insulin secretion from the islet -cell (Kowluru A. Diabetologia 44: 89-94, 2001; Kowluru A, Tannous M, and Chen HQ. Arch Biochem Biophys 398: 160-169, 2002). In this context, we also characterized a novel, ATP- and GTP-sensitive protein histidine kinase in isolated -cells that catalyzed the histidine phosphorylation of islet (endogenous) proteins as well as exogenously added histone 4, and we implicated this kinase in the activation of islet endogenous G proteins (Kowluru A. Biochem Pharmacol 63: 2091-2100, 2002). In the present study, we describe abnormalities in ATP- or GTP-mediated histidine phosphorylation of nucleoside diphosphate kinase in islets derived from the Goto-Kakizaki (GK) rat, a model for non-insulin-dependent diabetes. Furthermore, we provide evidence for a marked reduction in the activities of ATP- or GTP-sensitive histidine kinases in GK rat islets. On the basis of these observations, we propose that alterations in protein histidine phosphorylation could contribute toward insulin-secretory abnormalities demonstrable in the diabetic islet.

pancreatic islet; nucleoside diphosphate kinase; GTP-binding proteins; insulin secretion

This article has been cited by other articles:

				A. Kowluru Regulatory roles for small G proteins in the pancreatic {beta}-cell: lessons from models of impaired insulin secretion Am J Physiol Endocrinol Metab, October 1, 2003; 285(4): E669 - E684. [Abstract] [Full Text] [PDF]