AJP - Endo Add DOIs to your references at manuscript stage!
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab 284: E138-E147, 2003. First published October 1, 2002; doi:10.1152/ajpendo.00303.2002
0193-1849/03 $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
284/1/E138    most recent
00303.2002v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Web of Science (11)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Theodoropoulos, C.
Right arrow Articles by Gascon-Barré, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Theodoropoulos, C.
Right arrow Articles by Gascon-Barré, M.
Vol. 284, Issue 1, E138-E147, January 2003

High sensitivity of rat hepatic vitamin D3-25 hydroxylase CYP27A to 1,25-dihydroxyvitamin D3 administration

Catherine Theodoropoulos, Christian Demers, Jean-Luc Petit, and Marielle Gascon-Barré

Centre de recherche, Hôpital Saint-Luc, Centre Hospitalier de l'Université de Montréal, Département de Pharmacologie, Faculté de médecine, Université de Montréal, Montreal, Quebec, Canada H2X 1P1

CYP27A is considered the main vitamin D3 (D3)-25 hydroxylase in humans. Our purpose was to evaluate the effect of the D3 nutritional and hormonal status on hepatic CYP27A mRNA, cellular distribution, transcription rate, and enzyme activity. Studies were carried out in normal and in D-depleted rats supplemented with D3, 25OHD3, or 1,25(OH)2D3. CYP27A exhibited a significant gender difference and was observed throughout the hepatic acinus not only in hepatocytes but also in sinusoidal endothelial, stellate, and Kupffer cells. Neither D3 nor 25OHD3 influenced CYP27A mRNA levels. However, 1,25(OH)2D3 repletion led to a 60% decrease in CYP27A mRNA, which was accompanied by a 46% decrease in mitochondrial D3-25 hydroxylase activity. The effect of 1,25(OH)2D3 was mediated by a significant decrease in CYP27A transcription, whereas its mRNA half-life remained unchanged. Our data indicate that CYP27A is present in hepatic parenchymal and sinusoidal cells and that the gene transcript is not influenced by the D3 nutritional status but is transcriptionally regulated by 1,25(OH)2D3 exposure.

bile acid biosynthesis; Kupffer cells; stellate cells; hepatocytes; sinusoidal endothelial cells


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
M. Ellfolk, M. Norlin, K. Gyllensten, and K. Wikvall
Regulation of Human Vitamin D3 25-Hydroxylases in Dermal Fibroblasts and Prostate Cancer LNCaP Cells
Mol. Pharmacol., June 1, 2009; 75(6): 1392 - 1399.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
G. S Bevelander, E. S L C Pinto, A. V M Canario, T. Spanings, and G. Flik
CYP27A1 expression in gilthead sea bream (Sparus auratus, L.): effects of calcitriol and parathyroid hormone-related protein
J. Endocrinol., March 1, 2008; 196(3): 625 - 635.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Visit Other APS Journals Online