AJP - Endo Fuel your research with LabChart
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
 QUICK SEARCH:   [advanced]


     


Am J Physiol Endocrinol Metab (April 22, 2008). doi:10.1152/ajpendo.00774.2007
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
295/1/E70    most recent
00774.2007v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Beelen, M.
Right arrow Articles by van Loon, L. J. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Beelen, M.
Right arrow Articles by van Loon, L. J. C.
Submitted on December 13, 2007
Accepted on April 22, 2008

Protein co-ingestion stimulates muscle protein synthesis during resistance type exercise

Milou Beelen1*, Rene Koopman2, Annemie P. Gijsen3, Hanne Vandereyt4, Arie K Kies5, Harm Kuipers2, Wim H.M. Saris6, and Luc J. C. van Loon7

1 Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, Netherlands; , Netherlands
2 Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, Netherlands
3 Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands; Stable Isotope Research Center, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands
4 Stable Isotope Research Center, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands
5 DSM Food Specialties, Delft, Netherlands
6 Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands
7 Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, Netherlands; Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands; Stable Isotope Research Center, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Netherlands

* To whom correspondence should be addressed. E-mail: Milou.Beelen{at}BW.unimaas.nl.

In contrast to the impact of nutritional intervention on post-exercise muscle protein synthesis, little is known about the potential to modulate protein synthesis during exercise. This study investigates the impact of protein co-ingestion with carbohydrate on muscle protein synthesis during resistance type exercise. Ten healthy males were studied in the evening after consuming a standardized diet throughout the day. Subjects participated in 2 experiments, in which they ingested either carbohydrate or carbohydrate with protein during a 2h resistance exercise session. Subjects received a bolus of test drink prior to and every 15 min during exercise, providing 0.15 g·kg-1·h-1 carbohydrate with (CHO+PRO) or without (CHO) 0.15 g·kg-1·h-1 protein hydrolysate. Continuous intravenous infusions with L-[ring-13C6]phenylalanine and L-[ring-2H2] tyrosine were applied, and blood and muscle biopsies were collected to assess whole-body and muscle protein synthesis rates during exercise. Protein co-ingestion lowered whole-body protein breakdown rates by 8.4±3.6% (P=0.066), compared to the ingestion of carbohydrate only, and augmented protein oxidation and synthesis rates by 77±17 and 33±3%, respectively (P<0.01). As a consequence, whole-body net protein balance was negative in CHO, whereas a positive net balance was achieved following the CHO+PRO treatment (-4.4±0.3 vs 16.3±0.4 µmol phe·kg-1·h-1, respectively; P<0.01). In accordance, mixed muscle protein fractional synthetic rate (FSR) was 49±22% higher following protein co-ingestion (0.088±0.012 and 0.060±0.004 %·h-1 in CHO+PRO vs CHO treatment, respectively; P<0.05). We conclude that, even in a fed state, protein co-ingestion stimulates whole-body and muscle protein synthesis rates during resistance type exercise.




This article has been cited by other articles:


Home page
J. Nutr.Home page
M. Beelen, M. Tieland, A. P. Gijsen, H. Vandereyt, A. K. Kies, H. Kuipers, W. H. M. Saris, R. Koopman, and L. J. C. van Loon
Coingestion of Carbohydrate and Protein Hydrolysate Stimulates Muscle Protein Synthesis during Exercise in Young Men, with No Further Increase during Subsequent Overnight Recovery
J. Nutr., November 1, 2008; 138(11): 2198 - 2204.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2008 by the American Physiological Society.