Glucose transport is regarded as the principal rate control step governing insulin-stimulated glucose utilization by skeletal muscle. To assess this step in human skeletal muscle, quantitative PET imaging of skeletal muscle was performed using [¹¹C]3-OMG in healthy volunteers during a two-step insulin infusion (n=8; 30 and 120 mU/min-m², LO and HI) and during basal conditions (n=8). PET images were co-registered with MRI to assess [¹¹C]3-OMG activity in regions of interest placed on oxidative (soleus) compared to glycolytic (tibialis anterior) muscle. Insulin dose-responsive increases of [¹¹C]3-OMG activity in muscle were observed (p<0.01). Tissue activity was greater in soleus than in tibialis anterior (p<0.05). Spectral analysis identified that two mathematical components interacted to shape tissue activity curves. These two components were interpreted physiologically as likely representing the kinetics of [¹¹C]3-OMG delivery from plasma to tissue, and the kinetics of bi-directional glucose transport. During LO as compared to BASAL, there was a 6-fold increase in k₃, the rate constant attributed to inward glucose transport, and another 3-fold increase during HI (0.012±0.003 min^-1, 0.070±0.014 min^-1, 0.272±0.059 min^-1; p<0.001). Values for k₃ were similar in soleus and tibialis anterior, suggesting similar kinetics for transport, but compartmental modeling indicated a higher value in soleus for k₁, denoting higher rates of [¹¹C]3-OMG delivery to soleus than to tibialis anterior. In summary, in healthy volunteers there is robust dose-responsive insulin stimulation of glucose transport in skeletal muscle.