| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands
2 School of Exercise and Nutrition Sciences, Deakin University, Burwood, Victoria, Australia
3 Department of Human Biology, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands; Department of Movement Sciences, Nutrition and Toxicology Research Institute Maastricht (NUTRIM), Maastricht University, Maastricht, The Netherlands
* To whom correspondence should be addressed. E-mail: R.Koopman{at}Hb.unimaas.nl.
To investigate the in vivo effects of resistance exercise on translational control in human skeletal muscle, we determined the phosphorylation of AMP activated kinase (AMPK), eukaryotic initiation factor 4E-binding protein (4E-BP1), p70/p85-S6 protein kinase (S6K1) and ribosomal S6 protein (S6). Furthermore, we investigated whether changes in the phosphorylation of S6K1 are muscle fiber-type specific. Eight male subjects performed a single high-intensity resistance exercise session. Muscle biopsies were collected before, immediately after exercise, and following 30 and 120 min of post-exercise recovery. The phosphorylation status of AMPK, 4E-BP1, S6K1 and S6 was determined by western blotting with phospho-specific and pan antibodies. To determine fiber-type specific changes in the phosphorylation status of S6K1, (immuno)fluorescence microscopy was applied. AMPK phosphorylation was increased ~3 fold immediately following resistance exercise, while 4E-BP1 phosphorylation was reduced to 27±6% of pre-exercise values. Phosphorylation of S6K1 at Thr421/Ser424 was increased 2-2.5 fold during recovery, but did not induce a significant change in S6 phosphorylation. Phosphorylation of S6K1 was more pronounced in the type II vs. type I muscle fibers. Before exercise, phosphorylated S6K1 was predominantly located in the nuclei. After 2 h of post-exercise recovery, phospho-S6K1 was primarily located in the cytosol of type II muscle fibers. We conclude that resistance exercise effectively increases the phosphorylation of S6K1 on Thr421/Ser424, which is not associated with a substantial increase in S6 phosphorylation in a fasted state.
This article has been cited by other articles:
|
|
 |
|
  N. A. Burd, J. E. Tang, D. R. Moore, and S. M. Phillips Exercise training and protein metabolism: influences of contraction, protein intake, and sex-based differences J Appl Physiol, May 1, 2009; 106(5): 1692 - 1701. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Rose and E. A. Richter Regulatory mechanisms of skeletal muscle protein turnover during exercise J Appl Physiol, May 1, 2009; 106(5): 1702 - 1711. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tannerstedt, W. Apro, and E. Blomstrand Maximal lengthening contractions induce different signaling responses in the type I and type II fibers of human skeletal muscle J Appl Physiol, April 1, 2009; 106(4): 1412 - 1418. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
V. G. Coffey, H. Pilegaard, A. P. Garnham, B. J. O'Brien, and J. A. Hawley Consecutive bouts of diverse contractile activity alter acute responses in human skeletal muscle J Appl Physiol, April 1, 2009; 106(4): 1187 - 1197. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B. Verdijk, B. G. Gleeson, R. A. M. Jonkers, K. Meijer, H. H. C. M. Savelberg, P. Dendale, and L. J. C. van Loon Skeletal Muscle Hypertrophy Following Resistance Training Is Accompanied by a Fiber Type-Specific Increase in Satellite Cell Content in Elderly Men J Gerontol A Biol Sci Med Sci, March 1, 2009; 64A(3): 332 - 339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. B Verdijk, R. A. Jonkers, B. G Gleeson, M. Beelen, K. Meijer, H. H. Savelberg, W. K. Wodzig, P. Dendale, and L. J. van Loon Protein supplementation before and after exercise does not further augment skeletal muscle hypertrophy after resistance training in elderly men Am. J. Clinical Nutrition, February 1, 2009; 89(2): 608 - 616. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. J. Rose, B. Bisiani, B. Vistisen, B. Kiens, and E. A. Richter Skeletal muscle eEF2 and 4EBP1 phosphorylation during endurance exercise is dependent on intensity and muscle fiber type Am J Physiol Regulatory Integrative Comp Physiol, February 1, 2009; 296(2): R326 - R333. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Kumar, A. Selby, D. Rankin, R. Patel, P. Atherton, W. Hildebrandt, J. Williams, K. Smith, O. Seynnes, N. Hiscock, et al. Age-related differences in the dose-response relationship of muscle protein synthesis to resistance exercise in young and old men J. Physiol., January 1, 2009; 587(1): 211 - 217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. I. Glover, B. R. Oates, J. E. Tang, D. R. Moore, M. A. Tarnopolsky, and S. M. Phillips Resistance exercise decreases eIF2B{varepsilon} phosphorylation and potentiates the feeding-induced stimulation of p70S6K1 and rpS6 in young men Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2008; 295(2): R604 - R610. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Wilkinson, S. M. Phillips, P. J. Atherton, R. Patel, K. E. Yarasheski, M. A. Tarnopolsky, and M. J. Rennie Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle J. Physiol., August 1, 2008; 586(15): 3701 - 3717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Beelen, R. Koopman, A. P. Gijsen, H. Vandereyt, A. K. Kies, H. Kuipers, W. H. M. Saris, and L. J. C. van Loon Protein coingestion stimulates muscle protein synthesis during resistance-type exercise Am J Physiol Endocrinol Metab, July 1, 2008; 295(1): E70 - E77. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Morrison, D. Hara, Z. Ding, and J. L. Ivy Adding protein to a carbohydrate supplement provided after endurance exercise enhances 4E-BP1 and RPS6 signaling in skeletal muscle J Appl Physiol, April 1, 2008; 104(4): 1029 - 1036. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Deldicque, P. Atherton, R. Patel, D. Theisen, H. Nielens, M. J. Rennie, and M. Francaux Effects of resistance exercise with and without creatine supplementation on gene expression and cell signaling in human skeletal muscle J Appl Physiol, February 1, 2008; 104(2): 371 - 378. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Fujita, T. Abe, M. J. Drummond, J. G. Cadenas, H. C. Dreyer, Y. Sato, E. Volpi, and B. B. Rasmussen Blood flow restriction during low-intensity resistance exercise increases S6K1 phosphorylation and muscle protein synthesis J Appl Physiol, September 1, 2007; 103(3): 903 - 910. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Koopman, B. Pennings, A. H. G. Zorenc, and L. J. C. van Loon Protein Ingestion Further Augments S6K1 Phosphorylation in Skeletal Muscle Following Resistance Type Exercise in Males J. Nutr., August 1, 2007; 137(8): 1880 - 1886. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. F. Howlett, K. Sakamoto, A. Garnham, D. Cameron-Smith, and M. Hargreaves Resistance Exercise and Insulin Regulate AS160 and Interaction With 14-3-3 in Human Skeletal Muscle Diabetes, June 1, 2007; 56(6): 1608 - 1614. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. C. Dreyer, S. Fujita, J. G. Cadenas, D. L. Chinkes, E. Volpi, and B. B. Rasmussen Resistance exercise increases AMPK activity and reduces 4E-BP1 phosphorylation and protein synthesis in human skeletal muscle J. Physiol., October 15, 2006; 576(2): 613 - 624. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
