| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Neuroendocrine and Obesity Biology Unit, Liverpool Centre of Nutritional Genomics, School of Clinical Sciences, University of Liverpool, Liverpool, United Kingdom
2 The Henry Wellcome Laboratory of Molecular and Cellular Gastroenterology, School of Clinical Sciences, University of Liverpool, Liverpool, United Kingdom
* To whom correspondence should be addressed. E-mail: p.trayhurn{at}liverpool.ac.uk.
The expression profile of a series of adipokine genes linked to inflammation has been examined by quantitative PCR during the differentiation of human preadipocytes to adipocytes in primary culture, together with the integrated effects of TNF-
on the expression of these adipokines in the differentiated adipocytes. Expression of the genes encoding adiponectin, leptin and haptoglobin were highly differentiation-dependent, the mRNA being undetectable pre-differentiation with the level peaking 9-15 days post-differentiation. Although angiotensinogen (AGT) and monocyte chemoattractant protein-1 (MCP-1) were both expressed before differentiation, the mRNA level increased markedly on differentiation. The expression of nerve growth factor (NGF) and plasminogen activator inhibitor-1 (PAI-1) fell following differentiation, while that of TNF- and IL-6 changed little. Measurement of adiponectin, leptin, MCP-1 and NGF in the medium by ELISA showed that the protein secretion pattern paralleled cellular mRNA levels. Treatment of differentiated human adipocytes with TNF- (5 or 100 ng/ml for 24 h) significantly decreased the level of adiponectin, AGT and haptoglobin mRNA (by 2-4 fold) while that of leptin and PAI-1 was unchanged. In contrast, TNF- induced substantial increases in IL-6, TNF-, metallothionein, MCP-1 and NGF mRNAs, the largest increase being with MCP-1 (14.5-fold). MCP-1 and NGF secretion increased 8-10 fold with TNF-, while leptin and adiponectin did not change. These results demonstrate that there are major quantitative changes in adipokine gene expression during differentiation of human adipocytes and that TNF- has a pleiotropic effect on inflammation-related adipokine production, the synthesis of MCP-1 and NGF being highly induced by the cytokine.
This article has been cited by other articles:
|
|
 |
|
  B. Wang, I S. Wood, and P. Trayhurn Hypoxia induces leptin gene expression and secretion in human preadipocytes: differential effects of hypoxia on adipokine expression by preadipocytes J. Endocrinol., July 1, 2008; 198(1): 127 - 134. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M L. Trirogoff, A. Shintani, J. Himmelfarb, and T A. Ikizler Body mass index and fat mass are the primary correlates of insulin resistance in nondiabetic stage 3 4 chronic kidney disease patients Am. J. Clinical Nutrition, December 1, 2007; 86(6): 1642 - 1648. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S.-A. Lee, A. Kallianpur, Y.-B. Xiang, W. Wen, Q. Cai, D. Liu, S. Fazio, M. F. Linton, W. Zheng, and X. O. Shu Intra-individual Variation of Plasma Adipokine Levels and Utility of Single Measurement of These Biomarkers in Population-Based Studies Cancer Epidemiol. Biomarkers Prev., November 1, 2007; 16(11): 2464 - 2470. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. R. Zhou, E.-K. Kim, H. Kim, and K. J. Claycombe Obesity-associated mouse adipose stem cell secretion of monocyte chemotactic protein-1 Am J Physiol Endocrinol Metab, November 1, 2007; 293(5): E1153 - E1158. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Hausman, C. R. Barb, and R. G. Dean Patterns of gene expression in pig adipose tissue: Transforming growth factors, interferons, interleukins, and apolipoproteins J Anim Sci, October 1, 2007; 85(10): 2445 - 2456. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Bullo, M. R Peeraully, P. Trayhurn, J Folch, and J. Salas-Salvado Circulating nerve growth factor levels in relation to obesity and the metabolic syndrome in women Eur. J. Endocrinol., September 1, 2007; 157(3): 303 - 310. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Lo, L. E. Bernstein, B. Canavan, M. Torriani, M. B. Jackson, R. S. Ahima, and S. K. Grinspoon Effects of TNF-{alpha} neutralization on adipocytokines and skeletal muscle adiposity in the metabolic syndrome Am J Physiol Endocrinol Metab, July 1, 2007; 293(1): E102 - E109. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Vona-Davis and D. P Rose Adipokines as endocrine, paracrine, and autocrine factors in breast cancer risk and progression Endocr. Relat. Cancer, June 1, 2007; 14(2): 189 - 206. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J Simons, P. S van den Pangaart, J. M F G Aerts, and L. Boon Pro-inflammatory delipidizing cytokines reduce adiponectin secretion from human adipocytes without affecting adiponectin oligomerization J. Endocrinol., February 1, 2007; 192(2): 289 - 299. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M.-C. Alessi and I. Juhan-Vague PAI-1 and the Metabolic Syndrome: Links, Causes, and Consequences Arterioscler. Thromb. Vasc. Biol., October 1, 2006; 26(10): 2200 - 2207. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Trayhurn, C. Bing, and I. S. Wood Adipose Tissue and Adipokines--Energy Regulation from the Human Perspective J. Nutr., July 1, 2006; 136(7): 1935S - 1939S. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Krogh-Madsen, P. Plomgaard, K. Moller, B. Mittendorfer, and B. K. Pedersen Influence of TNF-{alpha} and IL-6 infusions on insulin sensitivity and expression of IL-18 in humans Am J Physiol Endocrinol Metab, July 1, 2006; 291(1): E108 - E114. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. T. Antunes, A. Gagnon, B. Chen, F. Pacini, T. J. Smith, and A. Sorisky Interleukin-6 release from human abdominal adipose cells is regulated by thyroid-stimulating hormone: effect of adipocyte differentiation and anatomic depot Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1140 - E1144. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. E. Bernstein, J. Berry, S. Kim, B. Canavan, and S. K. Grinspoon Effects of Etanercept in Patients With the Metabolic Syndrome. Arch Intern Med, April 24, 2006; 166(8): 902 - 908. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. B. Di Gregorio, A. Yao-Borengasser, N. Rasouli, V. Varma, T. Lu, L. M. Miles, G. Ranganathan, C. A. Peterson, R. E. McGehee, and P. A. Kern Expression of CD68 and Macrophage Chemoattractant Protein-1 Genes in Human Adipose and Muscle Tissues: Association With Cytokine Expression, Insulin Resistance, and Reduction by Pioglitazone Diabetes, August 1, 2005; 54(8): 2305 - 2313. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Dietze-Schroeder, H. Sell, M. Uhlig, M. Koenen, and J. Eckel Autocrine Action of Adiponectin on Human Fat Cells Prevents the Release of Insulin Resistance-Inducing Factors Diabetes, July 1, 2005; 54(7): 2003 - 2011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Bullo, M. R. Peeraully, and P. Trayhurn Stimulation of NGF expression and secretion in 3T3-L1 adipocytes by prostaglandins PGD2, PGJ2, and {Delta}12-PGJ2 Am J Physiol Endocrinol Metab, July 1, 2005; 289(1): E62 - E67. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. A. Nordstrom, M. Ryden, E. C. Backlund, I. Dahlman, M. Kaaman, L. Blomqvist, B. Cannon, J. Nedergaard, and P. Arner A Human-Specific Role of Cell Death-Inducing DFFA (DNA Fragmentation Factor-{alpha})-Like Effector A (CIDEA) in Adipocyte Lipolysis and Obesity Diabetes, June 1, 2005; 54(6): 1726 - 1734. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. H. Berg and P. E. Scherer Adipose Tissue, Inflammation, and Cardiovascular Disease Circ. Res., May 13, 2005; 96(9): 939 - 949. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
