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Articles in PresS, published online ahead of print March 12, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00475.2001
Submitted on October 22, 2001
Accepted on March 6, 2002
1 School of Health Sciences, Deakin University, Burwood, Victoria, Australia
2 Metabolic Research Unit, Deakin University, Geelong, Victoria, Australia
3 Department of Kinesiology, University of Waterloo, Waterloo, Ontario, Canada
* To whom correspondence should be addressed. E-mail: davidcs{at}deakin.edu.au.
The effects of a single bout of exercise and exercise training on the expression of genes necessary for the transport and ß-oxidation of fatty acids (FA), together with the gene expression of transcription factors implicated in the regulation of FA homeostasis were investigated. Seven human subjects (3 male, 4 female, 28.9 ± 3.1 yr of age, range 20-42 yr, BMI 22.6 kg/m2, 17-26 kg/m2 underwent a 9 day exercise training program of 60 min cycling per day at 63% VO2 peak (104 ± 14 Watts). On days 1 and 9 of the program, muscle biopsies were sampled from the vastus lateralis at rest, at the completion of exercise and again 3 hours post-exercise. Gene expression of key components of FA transport (FAT/CD36, FABPpm), ß-oxidation (CPT I, ß-HAD) and transcriptional control (PPAR
, PPAR
, PGC-1, SREBP-1c) were unaltered by exercise when measured at the completion and at 3 hours post-exercise. Training increased total lipid oxidation by 24% (p<0.05) for the 1 hour cycling bout. This increased capacity for lipid oxidation was accompanied by an increased expression of FAT/CD36 and CPT I mRNA. Similarly, FAT/CD36 protein abundance was also up-regulated by exercise training. We conclude that enhanced fat oxidation following exercise training is most closely associated with the genes involved in regulating fatty acid uptake across the plasma membrane (FAT/CD36) and across the mitochondrial membrane (CPT I).
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