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Am J Physiol Endocrinol Metab (March 18, 2003). doi:10.1152/ajpendo.00469.2002
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Submitted on October 30, 2002
Accepted on March 11, 2003

Contribution of plasma proteins to splanchnic and total anabolic utilization of dietary nitrogen in humans

Helene Fouillet1*, Claire Gaudichon1, Cecile Bos1, Francois Mariotti1, and Daniel Tome1

1 Institut National Agronomique, UMR INRA-INAPG de Physiologie de la Nutrition et du Comportement Alimentaire, Paris, France

* To whom correspondence should be addressed. E-mail: fouillet{at}inapg.inra.fr.

Splanchnic tissues are largely involved in the postprandial utilization of dietary amino acids, but little is yet known, particularly in humans, about the relative contributions of different splanchnic protein pools to splanchnic and total postprandial anabolism. Our aim was to develop a compartmental model that could distinguish between dietary nitrogen (N) incorporation into splanchnic constitutive, plasma (splanchnic exported) and peripheral proteins after a mixed protein meal in humans. Eight healthy subjects were fed a single mixed meal containing 15N-labeled soy protein, and dietary N postprandial kinetics were measured in plasma free amino acids, proteins and urea, and urinary urea and ammonia. These experimental data and others previously obtained for dietary N kinetics in ileal effluents under similar experimental conditions were used to develop the compartmental model. Six hours after the mixed meal ingestion, 31.5%, 7.5% and 21% of ingested N was predicted to be incorporated into splanchnic constitutive, splanchnic exported and peripheral proteins, respectively. The contribution of splanchnic exported proteins to total splanchnic anabolism from dietary N was predicted to be about 19% and to remain steady throughout the simulation period. Model behavior and its predictions were strongly in line with current knowledge of the system and the scarce, specific data available in the literature. This model provides the first data concerning the anabolism of splanchnic constitutive proteins in the non-steady postprandial state in humans. Using little invasive techniques, this model could help to assess how the splanchnic anabolism is modulated under different nutritional or physiopathological conditions in humans.




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