| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 The Rowett Research Institute, Aberdeen, Scotland, United Kingdom
* To whom correspondence should be addressed. E-mail: david.torrallardona{at}irta.es.
This study used a digesta transfer protocol to determine the site of absorption of lysine synthesized by the gastrointestinal microflora of pigs. Eight pigs were used, four with re-entrant cannulae in the terminal ileum, two with simple T-cannulae in the terminal ileum and two intact. All pigs were given, for five days, the same low protein diet that included fermentable carbohydrates. The diet of two pigs with re-entrant cannulae (donor) and of the two intact (control) pigs was supplemented with 15NH4Cl. The two other pigs with re-entrant cannulae (acceptor) pigs and those with simple cannulae (used to supply unlabelled digesta) were given the same diet but unlabelled. Ileal digesta were collected continuously from all the re-entrant cannulae and kept on ice. All digesta from each donor pig were reheated and returned to the distal cannula of its companion acceptor, whose ileal digesta were discarded. Unlabelled ileal digesta from the pigs with simple cannulae were instilled into the distal cannulae of the donor pigs. At the end of the experiment, the average 15N-enrichment in the plasma free lysine of control pigs was 0.0407 atom% excess, that of donor pigs was 0.0322 (79% of controls) whereas that of acceptor pigs was only 0.0096 (24% of controls). Due to nitrogen recycling, acceptor pigs had labeled lysine in the digesta of the stomach and small intestine, and donor pigs had labeled lysine in the digesta of the large intestine. If account is taken of the higher 15N-enrichment of microbial lysine in the large intestine, compared to the small intestine, it can be estimated that over 90% of the absorption of microbial lysine took place in the small intestine.
This article has been cited by other articles:
|
|
 |
|
  A. J. O. Libao-Mercado, C. L. Zhu, J. P. Cant, H. Lapierre, J.-N. Thibault, B. Seve, M. F. Fuller, and C. F. M. de Lange Dietary and Endogenous Amino Acids Are the Main Contributors to Microbial Protein in the Upper Gut of Normally Nourished Pigs J. Nutr., June 1, 2009; 139(6): 1088 - 1094. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Raj, U. Dileep, M. Vaz, M. F. Fuller, and A. V. Kurpad Intestinal Microbial Contribution to Metabolic Leucine Input in Adult Men J. Nutr., November 1, 2008; 138(11): 2217 - 2221. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Tome and C. Bos Lysine Requirement through the Human Life Cycle J. Nutr., June 1, 2007; 137(6): 1642S - 1645S. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Nieves Jr, H. S. Sitren, K. A. Herrlinger-Garcia, and B. Langkamp-Henken Pharmacologic Levels of Dietary Arginine in CB6F1 Mice Increase Serum Ammonia in the Healthy State and Serum Nitrite in Endotoxemia JPEN J Parenter Enteral Nutr, March 1, 2007; 31(2): 101 - 108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. Stoll and D. G. Burrin Measuring splanchnic amino acid metabolism in vivo using stable isotopic tracers J Anim Sci, April 1, 2006; 84(13_suppl): E60 - E. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
