AJP - Endo Ad Instruments
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab (June 18, 2002). doi:10.1152/ajpendo.00449.2001
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
283/4/E631    most recent
00449.2001v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via Web of Science (2)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Calsbeek, D. J.
Right arrow Articles by Hickey, M. S.
Right arrow Search for Related Content
PubMed
Right arrow Articles by Calsbeek, D. J.
Right arrow Articles by Hickey, M. S.

Articles in PresS, published online ahead of print June 18, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00449.2001
Submitted on October 5, 2001
Accepted on June 12, 2002

Metabolic and anthropometric factors related to skeletal muscle UCP-3 gene expression in healthy human adults

Dean J. Calsbeek1*, Traci L. Thompson2, Jennifer A Dahl2, Nicole R. Stob3, Joseph T. Brozinick, Jr.4, James O. Hill5, and Matt S. Hickey6

1 Physiology, Colorado State University, Fort Collins, CO, USA
2 Health and Exercise Science, Colorado State University, Fort Collins, CO, USA
3 Food Science and Human Nutrition, Colorado State University, Fort Collins, CO, USA
4 Lilly Corporate Center, Eli Lilly Co, Indianapolis, IN, USA
5 Center for Human Nutrition, University of Colorado Health Science Center, Denver, CO, USA
6 Health and Exercise Science, Colorado State University, Fort Collins, CO, USA; Physiology, Colorado State University, Fort Collins, CO, USA; Food Science and Human Nutrition, Colorado State University, Fort Collins, CO, USA

* To whom correspondence should be addressed. E-mail: dean.calsbeek{at}cahs.colostate.edu.

This cross-sectional investigation sought to determine the relationship between selected metabolic, endocrine, and anthropometric factors and skeletal muscle UCP3mRNA in healthy adult humans. Twenty-four healthy adults (13M, 11F) across a range of aerobic capacity, age, and body composition were studied. Muscle biopsies were obtained from the vastus lateralis from which UCP3 mRNA was quantified by Northern blot and fiber type was determined using the Myosin ATPase staining procedure. In addition, resting energy expenditure (REE) and VO2max were determined by indirect calorimetry, body composition was determined by DEXA, and fasting plasma leptin and insulin were determined by ELISA. UCP3 mRNA was positively correlated with the % type I fibers (r=0.842, P<0.001), plasma leptin (r=0.454, P=0.026), plasma insulin (r=0.615, P<0.001), and inversely to age (r=-0.411, P=0.046). Stepwise multiple regression analysis determined percent type I muscle fibers was the best predictor of vastus lateralis UCP3 mRNA, and no other variable entered the equation (model r2 = 0.66). This study suggests that of the variables measured, UCP3 mRNA is primarily related to skeletal muscle fiber type in healthy adults. The factors that contribute to fiber-specific differences in UCP3 mRNA expression will need to be examined in future studies.






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2002 by the American Physiological Society.