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Am J Physiol Endocrinol Metab (December 13, 2005). doi:10.1152/ajpendo.00444.2005
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Submitted on September 13, 2005
Accepted on December 5, 2005

Effects of streptozotocin-induced diabetes and physical training on gene expression of extracellular matrix proteins in mouse skeletal muscle

T. Maarit Lehti1*, Mika Silvennoinen2, Riikka Kivela2, Heikki Kainulainen3, and Jyrki Komulainen2

1 LIKES Research Center for Sport and Health Sciences, Jyvaskyla, Finland
2 LIKES Research Center for Sport and Health Sciences, Jyvaskyla, Finland; Department of Biology of Physical Activity, University of Jyvaskyla, Neuromuscular Research Center, Jyvaskyla, Finland
3 Department of Biology of Physical Activity, University of Jyvaskyla, Neuromuscular Research Center, Jyvaskyla, Finland

* To whom correspondence should be addressed. E-mail: maarit.lehti{at}likes.fi.

Diabetes induces changes in the structure and function of the extracellular matrix (ECM) in many tissues. We investigated the effects of diabetes, physical training, and their combination on the gene expression of ECM proteins in skeletal muscle. Mice were divided to control (C), training (T), streptozotocin-induced diabetic (D), and diabetic training (DT) groups. Training groups (T, DT) performed 1, 3, or 5 weeks of endurance training on a treadmill. Gene expression of calf muscles was analyzed using microarray and quantitative PCR. Training group samples were collected 24 hours after the last training session. Diabetes affected the gene expression of several collagens (types I, III, IV, V, VI, and XV), some non-collagenous glycoproteins, and proteoglycans (e.g. elastin, thrombospondin-1, laminin-2, decorin). Reduced gene expression of collagens in diabetic skeletal muscle was partially attenuated as a result of physical training. In diabetes, mRNA expression of the basement membrane (BM) collagens decreased and that of non-collagenous glycoproteins increased. This may change the structure of the BM in a less collagenous direction and affect its properties.




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