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Am J Physiol Endocrinol Metab (March 12, 2002). doi:10.1152/ajpendo.00404.2001
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Articles in PresS, published online ahead of print March 12, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00404.2001
Submitted on September 12, 2001
Accepted on March 12, 2002

Effects of endurance training on activity and expression of AMP-activated protein kinase isoforms in rat muscles

Paula E Durante1, Kirsty J Mustard1, Soo-Hyun Park2, William W Winder2, and D. Grahame Hardie1*

1 Division of Molecular Physiology, Dundee University, Dundee, Scotland, United Kingdom
2 Department of Zoology, Brigham Young University, Provo, UT 84602, USA

* To whom correspondence should be addressed. E-mail: d.g.hardie{at}dundee.ac.uk.

The effects of endurance training on the response of muscle AMP-activated protein kinase (AMPK) and acetyl-CoA carboxylase (ACC) to moderate treadmill exercise were examined. In red quadriceps there was a large activation of {alpha}2-AMPK and inactivation of ACC in response to exercise. This response was greatly reduced after training, probably because of reduced metabolic stress. In white quadriceps there were no effects of exercise on AMPK or ACC, but {alpha}2 activity was higher after training, due to increased phosphorylation of Thr-172. In soleus there were small increases in {alpha}2 activity during exercise that were not affected by training. The expression of all seven AMPK subunit isoforms was also examined. The ß2 and {gamma}2 isoforms were most highly expressed in white quadriceps, and {gamma}3 in red quadriceps and soleus. There was a 3-fold increase in expression of {gamma}3 after training in red quadriceps only. Our results suggest that {gamma}3 might have a special role in the adaptation to endurance exercise in muscles utilizing oxidative metabolism.




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