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Articles in PresS, published online ahead of print November 19, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00381.2002
Submitted on August 29, 2002
Accepted on November 15, 2002
1 Department of Obstetrics, Gynecology and Women's Health, Albert Einstein College of Medicine, Division of Reproductive Endocrinology, Bronx, New York, USA
2 Department of Medicine, University of Massachusetts Medical School, Division of Preventive and Behavioral Medicine, Worcester, Massachusetts, USA; New England Research Institutes, Watertown, Massachusetts, USA
3 Center for Gerontology and Health Care Research, Brown Medical School, Providence, Rhode Island, USA; New England Research Institutes, Watertown, Massachusetts, USA
4 Department of Epidemiology and Preventive Medicine, University of California, Davis, California, USA
5 Division of Geriatrics, University of California, Los Angeles, California, USA
6 Center for Health Sciences, University of California, Los Angeles, California, USA
7 Department of Population Health and Reproduction, University of California, Davis, California, USA
8 Reproductive Sciences Program, University of Michigan, Ann Arbor, Michigan, USA
9 Harvard Injury Control Research Center, Harvard University, Boston, Massachusetts, USA; New England Research Institutes, Watertown, Massachusetts, USA
10 Department of Epidemiology, University of Michigan, Ann Arbor, Michigan, USA
11 Department of Obstetrics, Gynecology and Women's Health, University of Medicine and Dentistry of New Jersey, Newark, New Jersey, USA
* To whom correspondence should be addressed. E-mail: Glicktoro{at}aol.com.
The Study of Women's Health Across the Nation (SWAN) is a multi-ethnic cohort study of middle aged women enrolled at seven sites throughout the nation. A subset of the SWAN sample (n=848 women) completed a substudy in which their excretion of gonadotropins and sex steroid metabolites was assessed through the collection of first-morning voided urine samples during one complete menstrual cycle or 50 consecutive days (whichever came first). Urine was analyzed for LH, FSH, estrone conjugates (E1c) and prenanediol glucuronide (Pdg), using newly developed chemiluminescent assays. An annual collection is planned for all of the women who participated in the baseline urine collection protocol. In order to prepare for the analysis of this large database of daily menstrual cycle hormone data in a population of reproductively aging women, we examined the performance of algorithms designed to identify features of the normal menstrual cycle, to determine whether the algorithms could be applied to these women's cycles as they continue through the menopausal transition. Algorithms were basedon existing methods, and were compared with a "gold standard" of ratings of trained observers on a subset of 396 cycles from the first collection of Daily Hormone Study samples. For purposes of comparison, similar analyses were done on cycles from a group of 29 mid-reproductive aged women.
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