In order to detect whether the mutual interaction occurring between the osteocytes-bone lining cells system (OBLCS) and the bone extracellular fluid (BECF), filling the lacuno-canalicular network, is affected by load through a modification of the BECF-ECF (systemic extracellular fluid) gradient, metatarsal bones of weanling mice, immersed in ECF, were subjected ex vivo to a 2' cyclic axial load by respectively varying the load parameters (amplitude and frequency). The electric (ionic) currents at the bone surface were measured by means of a vibrating probe after having exposed BECF to ECF through a transcortical hole drilled across the bone cortex.. The application of load increased the ionic current at the site of damage in a dose dependent manner. The post load current density subsequently decayed following an exponential pattern. Post-load increment's amplitude and subsequent decay were quantitatively and qualitatively dependent upon bone viability. Dummy and static loads did not induce current density modifications. Since BECF is perturbed by loading, it is conceivable that OBLCS tends to restore BECF preload conditions by controlling ion fluxes at the bone-plasma interface to fulfill metabolic needs. Since the electric current reflects the integrated activity of OBLCS, its evaluation in transgenic mice engineered to possess genetic lesions in channels or matrix constituents could be helpful in the characterization of the mechanical and metabolic functions of bone.