The present study investigated potential sex differences in the metabolic response to carbohydrate (CHO) ingestion during exercise. Moderately endurance trained men and women (n=8 for each sex) performed 2 h cycling at ~67% (VO²max) without (water, WAT) and with CHO ingestion (1.5 g glucose per min). Substrate oxidation and kinetics were quantified during exercise using indirect calorimetry and stable isotope techniques (¹³C glucose ingestion, [6,6-²H₂]-glucose and [²H₅]-glycerol infusion). In both sexes, CHO ingestion significantly increased the rate of appearance (Ra) and disappearance (Rd) of glucose during exercise compared to WAT ingestion (males: WAT, ~28-29 µmol/kg lean body mass (LBM)/min, CHO, ~53 µmol/kg LBM/min; females: WAT, ~28-29 µmol/kg LBM/min, CHO, ~61 µmol/kg LBM/min; main effect of trial, P<0.05). The contribution of plasma glucose oxidation to the energy yield was significantly increased with CHO ingestion in both sexes (from ~10% to ~20% of energy expenditure, main effect of trial, P<0.05). Liver-derived glucose oxidation was reduced, while the rate of muscle glycogen oxidation was unaffected with CHO ingestion (males: WAT, 108±12 µmol/kg LBM/min, CHO, 108±11 µmol/kg LBM/min; females: WAT, 89±10 µmol/kg LBM/min, CHO, 93±11 µmol/kg LBM/min). CHO ingestion reduced fat oxidation and lipolytic rate (Ra glycerol) to a similar extent in both sexes. Finally, ingested CHO was oxidized at similar rates in men and women during exercise (peak rates of 0.70±0.08 and 0.65±0.06 g/min respectively). The present investigation demonstrates that the metabolic response to CHO ingestion during exercise is similar in men and women.