| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Division of Cell Biology, Department of Biomedicine and Surgery, Linkoping University, Linkoping, Sweden
2 Division of Cell Biology, Department of Biomedicine and Surgery, Linkoping University, Linkoping, Sweden; Division of Internal Medicine, Department of Medicine and Care, Faculty of Health Sciences, Linkoping University, Linkoping, Sweden
* To whom correspondence should be addressed. E-mail: hans.arnqvist{at}ibk.liu.se.
Aim. Micro- and macroangiopathy are major causes of morbidity and mortality in patients
with diabetes. Our aim was to characterize IGF-IR and IR in human micro- and
macrovascular endothelial cells.
Methods. Cultured human dermal microvascular endothelial cells (HMVEC) and human
aortic endothelial cells (HAEC) were used. Gene expression was measured by quantitative
real-time RT-PCR and receptor protein by ligand binding assay. Phosphorylation of IGF-IR
-subunit was analyzed by immunoprecipitation and Western blot. Glucose metabolism
and DNA synthesis was assessed using 3H-glucose and 3H-thymidine incorporation,
respectively.
Results. We detected gene expression of IGF-IR and IR in HAEC and HMVEC. IGF-IR
gene expression was several folds higher than that of IR. The specific binding of 125I-IGF-I
was higher than that of 125I-insulin in HAEC and HMVEC. Insulin and the new long acting
insulin analog, glargine, interacted with the IGF-IR with thousand and hundred fold less
potency than IGF-I itself. Phosphorylation of the IGF-IR -subunit was shown in HAEC
for IGF-I (10-8M) and insulin (10-6M) and in HMVEC for IGF-I and glargine (10-8M, 10-
6M). IGF-I 10-7M stimulated incorporation of 3H-thymidine into DNA, and at a
concentration of 10-9-10-7M also the incorporation of 3H-glucose in HMVEC, whereas
glargine and insulin had no significant effects at concentrations of 10-9-10-7M.
Conclusion. Human micro- and macrovascular endothelial cells express more IGF-IR than
IR. IGF-I and high concentrations of glargine and insulin activates the IGF-IR. Glargine
has a higher affinity than insulin for the IGF-IR, but probably has no effect on DNA
synthesis at concentrations reached in vivo.
This article has been cited by other articles:
|
|
 |
|
  S. Gogg, U. Smith, and P.-A. Jansson Increased MAPK Activation and Impaired Insulin Signaling in Subcutaneous Microvascular Endothelial Cells in Type 2 Diabetes: The Role of Endothelin-1 Diabetes, October 1, 2009; 58(10): 2238 - 2245. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Tanaka, L. Qiang, A. S. Banks, C. L. Welch, M. Matsumoto, T. Kitamura, Y. Ido-Kitamura, R. A. DePinho, and D. Accili Foxo1 Links Hyperglycemia to LDL Oxidation and Endothelial Nitric Oxide Synthase Dysfunction in Vascular Endothelial Cells Diabetes, October 1, 2009; 58(10): 2344 - 2354. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Potenza, F. Addabbo, and M. Montagnani Vascular actions of insulin with implications for endothelial dysfunction Am J Physiol Endocrinol Metab, September 1, 2009; 297(3): E568 - E577. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, A. X. Wang, Z. Liu, and E. J. Barrett Insulin Signaling Stimulates Insulin Transport by Bovine Aortic Endothelial Cells Diabetes, March 1, 2008; 57(3): 540 - 547. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Marini, E. Succurro, S. Frontoni, M. L. Hribal, F. Andreozzi, R. Lauro, F. Perticone, and G. Sesti Metabolically Healthy but Obese Women Have an Intermediate Cardiovascular Risk Profile Between Healthy Nonobese Women and Obese Insulin-Resistant Women Diabetes Care, August 1, 2007; 30(8): 2145 - 2147. [Full Text] [PDF]
|
|
|
|
 |
|
 Z. Wang, G. Chakravarty, S. Kim, Y. D. Yazici, M. N. Younes, S. A. Jasser, A. A. Santillan, C. D. Bucana, A. K. El-Naggar, and J. N. Myers Growth-Inhibitory Effects of Human Anti-Insulin-Like Growth Factor-I Receptor Antibody (A12) in an Orthotopic Nude Mouse Model of Anaplastic Thyroid Carcinoma Clin. Cancer Res., August 1, 2006; 12(15): 4755 - 4765. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Wang, Z. Liu, G. Li, and E. J. Barrett The vascular endothelial cell mediates insulin transport into skeletal muscle Am J Physiol Endocrinol Metab, August 1, 2006; 291(2): E323 - E332. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. M. van Golen, T. S. Schwab, B. Kim, M. E. Soules, S. Su Oh, K. Fung, K. L. van Golen, and E. L. Feldman Insulin-Like Growth Factor-I Receptor Expression Regulates Neuroblastoma Metastasis to Bone. Cancer Res., July 1, 2006; 66(13): 6570 - 6578. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. R. Gosmanov, F. B. Stentz, and A. E. Kitabchi De novo emergence of insulin-stimulated glucose uptake in human aortic endothelial cells incubated with high glucose Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E516 - E522. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
