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Am J Physiol Endocrinol Metab (October 18, 2005). doi:10.1152/ajpendo.00321.2005
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Submitted on July 18, 2005
Accepted on October 14, 2005

Increased mitochondrial glycerol-3-phosphate acyltransferase protein and enzyme activity in rat epididymal fat upon cessation of wheel running

David S Kump1, Matthew J Laye1, and Frank W Booth1*

1 Department of Medical Pharmacology and Physiology, University of Missouri, Columbia, MO, USA

* To whom correspondence should be addressed. E-mail: boothf{at}missouri.edu.

Triacylglycerol synthesis in rat epididymal fat overshoots sedentary levels at 10, 29, and 53 hours of physical inactivity after 21 days of wheel running. The purposes of the current study were to determine: 1) whether this effect is also observed after an acute bout of physical activity and 2) what enzymatic changes might contribute to this effect. We show that more than one bout of physical activity, such as occurs with 21 days of wheel running, is necessary for triacylglycerol synthesis to overshoot sedentary values, suggesting that pre-translational mechanisms may be responsible for this overshoot effect. Ten hours following 21 days of wheel running, activity of the mitochondrial glycerol-3-phosphate acyltransferase-1 isoform (mtGPAT1), a key regulator of triacylglycerol synthesis, overshot sedentary values by 48%, and remained higher than sedentary at 29 and 53 hours of reduced physical activity. The overshoot in mtGPAT1 activity was accompanied by an increase in mtGPAT protein level. Cyclic AMP response element binding protein binding protein level was higher in sedentary than 29 hours after 21 days of wheel running. Adenosine 5'-monophosphate-activated protein kinase-{alpha} Thr172 phosphorylation was increased immediately following treadmill running, but decreased to sedentary values by 5 hours post-activity. Casein kinase-2-{alpha} protein level and activity were unchanged. We conclude that an increase in mtGPAT protein may contribute to the overshoot in triacylglycerol synthesis.




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