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Articles in PresS, published online ahead of print September 17, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00321.2002
Submitted on July 17, 2002
Accepted on September 10, 2002
1 Department of Medicine, The University of Chicago, Chicago, IL, USA
2 Howard Hughes Medical Institute, Chicago, IL, USA
3 Department of Pathology, The University of Chicago, Chicago, IL, USA
4 Department of Neurobiology, Pharmacology and Physiology, The University of Chicago, Chicago, IL, USA
5 Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN, USA
6 Howard Hughes Medical Institute, Chicago, IL, USA; Department of Medicine, The University of Chicago, Chicago, IL, USA; Department of Biochemistry and Molecular Biology, The University of ChicagoHoward Hughes Medical Institute, Chicago, IL, USA
* To whom correspondence should be addressed. E-mail: mhara{at}midway.uchicago.edu.
We have generated transgenic mice that express green fluorescent protein (GFP) under the control of the mouse insulin I gene promoter (MIP). The MIP-GFP mice develop normally and are indistinguishable from control animals with respect to glucose tolerance and pancreatic insulin content. Histological studies showed that the MIP-GFP mice had normal islet architecture with co-expression of insulin and GFP in the ß-cells of all islets. We observed GFP expression in islets from embryonic E13.5 day through adult. Studies of ß-cell function revealed no difference in glucose-induced intracellular calcium mobilization between islets from transgenic and control animals. We prepared single-cell suspensions from both isolated islets and whole pancreas from MIP-GFP transgenic mice and sorted the ß-cells by fluorescence-activated cell sorting based on their green fluorescence. These studies showed that 2.4±0.2% (n=6) of the cells in the pancreas of newborn (P1) and 0.9±0.1% (n=5) of 8-week old mice were ß-cells. The MIP-GFP transgenic mice may be a useful tool for studying ß-cell biology in normal and diabetic animals.
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