| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Copenhagen Muscle Research Centre, Institute of Exercise and Sport Sciences, University of Copenhagen, Copenhagen, Denmark
2 Wellcome Trust Biocentre, Division of Molecular Physiology, Faculty of Life Sciences, University of Dundee, Dundee, Scotland, United Kingdom
* To whom correspondence should be addressed. E-mail: Cfrosig{at}ifi.ku.dk.
The 5'AMP-activated protein kinase (AMPK) is proposed to be involved in signaling
pathways leading to adaptations in skeletal muscle in response to both a single exercise
bout and exercise training. This study investigated the effect of endurance training on
protein content of catalytic (
1, 2) and regulatory (
1, 2 and 
1, 2, 3) subunit isoforms of
AMPK as well as on basal AMPK activity in human skeletal muscle. Eight young healthy
men performed supervised one-legged knee extensor endurance training for three weeks.
Muscle biopsies were obtained before and 15 hours after training in both legs. In
response to training the protein content of 1, 2 and 1 increased in the trained leg by
41%, 34% and 26%, respectively (1 and 2 P<0.005; 1 P<0.05). In contrast, the protein
content of the regulatory 3 isoform decreased by 62% in the trained leg (P=0.01), while no
effect of training was seen for 2, 1 and 2. AMPK activity associated with the 1 and the
2 isoforms increased in the trained leg by 94% and 49%, respectively (both, P<0.05 ). In
agreement with these observations, phosphorylation of -AMPK(Thr172) and of the known
AMPK target acetyl-CoA carboxylase-(Ser221) increased by 74% and 180%, respectively
(both, P<0.001). This study demonstrates that protein content and basal AMPK activity in
human skeletal muscle is highly susceptible to endurance exercise training. Except for the
increase in 1 protein, all observed adaptations to training could be ascribed to local
contraction-induced mechanisms, since they did not occur in the contra-lateral untrained
muscle.
This article has been cited by other articles:
|
|
 |
|
  N. Fujii, R. C. Ho, Y. Manabe, N. Jessen, T. Toyoda, W. L. Holland, S. A. Summers, M. F. Hirshman, and L. J. Goodyear Ablation of AMP-Activated Protein Kinase {alpha}2 Activity Exacerbates Insulin Resistance Induced by High-Fat Feeding of Mice Diabetes, November 1, 2008; 57(11): 2958 - 2966. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. B. Wilkinson, S. M. Phillips, P. J. Atherton, R. Patel, K. E. Yarasheski, M. A. Tarnopolsky, and M. J. Rennie Differential effects of resistance and endurance exercise in the fed state on signalling molecule phosphorylation and protein synthesis in human muscle J. Physiol., August 1, 2008; 586(15): 3701 - 3717. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. De Bock, W. Derave, B. O. Eijnde, M. K. Hesselink, E. Koninckx, A. J. Rose, P. Schrauwen, A. Bonen, E. A. Richter, and P. Hespel Effect of training in the fasted state on metabolic responses during exercise with carbohydrate intake J Appl Physiol, April 1, 2008; 104(4): 1045 - 1055. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Vissing, S. L. McGee, C. Roepstorff, P. Schjerling, M. Hargreaves, and B. Kiens Effect of sex differences on human MEF2 regulation during endurance exercise Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E408 - E415. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Richard-Bulteau, B. Serrurier, B. Crassous, S. Banzet, A. Peinnequin, X. Bigard, and N. Koulmann Recovery of skeletal muscle mass after extensive injury: positive effects of increased contractile activity Am J Physiol Cell Physiol, February 1, 2008; 294(2): C467 - C476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. D. Mason, H. Rundqvist, I. Papandreou, R. Duh, W. J. McNulty, R. A. Howlett, I. M. Olfert, C. J. Sundberg, N. C. Denko, L. Poellinger, et al. HIF-1{alpha} in endurance training: suppression of oxidative metabolism Am J Physiol Regulatory Integrative Comp Physiol, November 1, 2007; 293(5): R2059 - R2069. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. J. Rose, C. Frosig, B. Kiens, J. F. P. Wojtaszewski, and E. A. Richter Effect of endurance exercise training on Ca2+ calmodulin-dependent protein kinase II expression and signalling in skeletal muscle of humans J. Physiol., September 1, 2007; 583(2): 785 - 795. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. T. Putman, K. J. B. Martins, M. E. Gallo, G. D. Lopaschuk, J. A. Pearcey, I. M. MacLean, R. J. Saranchuk, and D. Pette {alpha}-Catalytic subunits of 5'AMP-activated protein kinase display fiber-specific expression and are upregulated by chronic low-frequency stimulation in rat muscle Am J Physiol Regulatory Integrative Comp Physiol, September 1, 2007; 293(3): R1325 - R1334. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. S. Chow, L. J. Greenlund, Y. W. Asmann, K. R. Short, S. K. McCrady, J. A. Levine, and K. S. Nair Impact of endurance training on murine spontaneous activity, muscle mitochondrial DNA abundance, gene transcripts, and function J Appl Physiol, March 1, 2007; 102(3): 1078 - 1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Freyssenet Energy sensing and regulation of gene expression in skeletal muscle J Appl Physiol, February 1, 2007; 102(2): 529 - 540. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. B. Jorgensen, J. T. Treebak, B. Viollet, P. Schjerling, S. Vaulont, J. F. P. Wojtaszewski, and E. A. Richter Role of AMPK{alpha}2 in basal, training-, and AICAR-induced GLUT4, hexokinase II, and mitochondrial protein expression in mouse muscle Am J Physiol Endocrinol Metab, January 1, 2007; 292(1): E331 - E339. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Ventura-Clapier, B. Mettauer, and X. Bigard Beneficial effects of endurance training on cardiac and skeletal muscle energy metabolism in heart failure Cardiovasc Res, January 1, 2007; 73(1): 10 - 18. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. B. Birk and J. F. P. Wojtaszewski Predominant {alpha}2/{beta}2/{gamma}3 AMPK activation during exercise in human skeletal muscle J. Physiol., December 15, 2006; 577(3): 1021 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Roepstorff, M. Thiele, T. Hillig, H. Pilegaard, E. A. Richter, J. F. P. Wojtaszewski, and B. Kiens Higher skeletal muscle {alpha}2AMPK activation and lower energy charge and fat oxidation in men than in women during submaximal exercise J. Physiol., July 1, 2006; 574(1): 125 - 138. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. J. Atherton and M. J. Rennie Protein synthesis a low priority for exercising muscle J. Physiol., June 1, 2006; 573(2): 288 - 289. [Full Text] [PDF]
|
|
|
|
|
|
J. E. Kuhl, N. B. Ruderman, N. Musi, L. J. Goodyear, M. E. Patti, S. Crunkhorn, D. Dronamraju, A. Thorell, J. Nygren, O. Ljungkvist, et al. Exercise training decreases the concentration of malonyl-CoA and increases the expression and activity of malonyl-CoA decarboxylase in human muscle Am J Physiol Endocrinol Metab, June 1, 2006; 290(6): E1296 - E1303. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sriwijitkamol, J. L. Ivy, C. Christ-Roberts, R. A. DeFronzo, L. J. Mandarino, and N. Musi LKB1-AMPK signaling in muscle from obese insulin-resistant Zucker rats and effects of training Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E925 - E932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Toyoda, S. Tanaka, K. Ebihara, H. Masuzaki, K. Hosoda, K. Sato, T. Fushiki, K. Nakao, and T. Hayashi Low-intensity contraction activates the {alpha}1-isoform of 5'-AMP-activated protein kinase in rat skeletal muscle Am J Physiol Endocrinol Metab, March 1, 2006; 290(3): E583 - E590. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shearer, R. J. Wilson, D. S. Battram, E. A. Richter, D. L. Robinson, M. Bakovic, and T. E. Graham Increases in glycogenin and glycogenin mRNA accompany glycogen resynthesis in human skeletal muscle Am J Physiol Endocrinol Metab, September 1, 2005; 289(3): E508 - E514. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. F. P Wojtaszewski, J. B Birk, C. Frosig, M. Holten, H. Pilegaard, and F. Dela 5'AMP activated protein kinase expression in human skeletal muscle: effects of strength training and type 2 diabetes J. Physiol., April 15, 2005; 564(2): 563 - 573. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. O. Eijnde, W. Derave, J. F. P. Wojtaszewski, E. A. Richter, and P. Hespel AMP kinase expression and activity in human skeletal muscle: effects of immobilization, retraining, and creatine supplementation J Appl Physiol, April 1, 2005; 98(4): 1228 - 1233. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Pold, L. S. Jensen, N. Jessen, E. S. Buhl, O. Schmitz, A. Flyvbjerg, N. Fujii, L. J. Goodyear, C. F. Gotfredsen, C. L. Brand, et al. Long-Term AICAR Administration and Exercise Prevents Diabetes in ZDF Rats Diabetes, April 1, 2005; 54(4): 928 - 934. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Roepstorff, N. Halberg, T. Hillig, A. K. Saha, N. B. Ruderman, J. F. P. Wojtaszewski, E. A. Richter, and B. Kiens Malonyl-CoA and carnitine in regulation of fat oxidation in human skeletal muscle during exercise Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E133 - E142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Taylor, D. Hurst, L. J. Greenwood, J. D. Lamb, T. D. Cline, S. N. Sudweeks, and W. W. Winder Endurance training increases LKB1 and MO25 protein but not AMP-activated protein kinase kinase activity in skeletal muscle Am J Physiol Endocrinol Metab, December 1, 2004; 287(6): E1082 - E1089. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. A. Clark, Z.-P. Chen, K. T. Murphy, R. J. Aughey, M. J. McKenna, B. E. Kemp, and J. A. Hawley Intensified exercise training does not alter AMPK signaling in human skeletal muscle Am J Physiol Endocrinol Metab, May 1, 2004; 286(5): E737 - E743. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
