Stearoyl-CoA desaturase (SCD) catalyzes the synthesis of oleate (C18:1) and palmitoleate (C16:1) which are the main monounsaturated fatty acids of membrane phospholipids (PL), triglycerides (TG), wax esters and cholesterol esters. Previously, we showed SCD1 deficiency elevates insulin signaling components and downregulates protein-tyrosine phosphatase 1B in muscle a major insulin sensitive tissue. Here we found that in the brown adipose tissue another insulin sensitive tissue, the basal tyrosine phosphorylation of the insulin receptor (IR), and insulin receptor substrates (IRS-1 and IRS-2) were upregulated in SCD1-/- mice compared to the wild-type mice. The association of IRS-1 and IRS-2 with p85 subunit of phosphatidylinositol 3-kinase as well as the Akt-Ser 473 and Akt-Thr 308 phosphorylation are also elevated in the SCD1- /- mice. The mRNA expression, protein levels, and activity of the protein-tyrosine phosphatase-1B (PTP-1B) implicated in the attenuation of the insulin signal, are reduced in the SCD1-/- mice. The content of glucose transporter 4 in the plasma membrane increased 2.5 fold and this was accompanied by a 6-fold increase in glucose uptake in BAT of SCD1-/- mice. The increased glucose uptake was associated with higher glycogen synthase activity and glycogen accumulation. In presence of insulin, [U- ¹⁴C]glucose incorporation into glycogen was increased in BAT of SCD1-/- mice. Taken together, these studies illustrate increased insulin signaling and increased glycogen metabolism in BAT of SCD1-/- mice.