| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center and Laval University, Quebec city, PQ, Canada; Department of Nutrition, Laval University Medical Research Center and Laval University, Quebec city, PQ, Canada
2 Molecular Endocrinology and Oncology Research Center, Laval University Medical Research Center and Laval University, Quebec city, PQ, Canada
3 Gynecology Unit, Laval University Medical Research Center and Laval University, Quebec city, PQ, Canada
* To whom correspondence should be addressed. E-mail: andre.tchernof{at}crchul.ulaval.ca.
We examined the expression and activity of steroid aldoketoreductase (AKR) 1C enzymes in
abdominal subcutaneous and omental adipose tissue in women. AKR1C1 (20
-hydroxysteroid
dehydrogenase), AKR1C2 (3-hydroxysteroid dehydrogenase type 3), and AKR1C3 (17
-
hydroxysteroid dehydrogenase type 5) are involved mainly in the conversion of progesterone to
20-hydroxyprogesterone and in the inactivation of dihydrotestosterone to 5-androstane-
3,17-diol. Abdominal subcutaneous (Sc) and omental (Om) adipose tissue biopsies were
obtained during abdominal hysterectomies in 7 women with low visceral adipose tissue area and
7 age- and total body fat mass-matched women with visceral obesity. Body composition and
body fat distribution were assessed before surgery by dual energy x-ray absorptiometry and
computed tomography. Women with elevated visceral adipose tissue areas were characterized by
significantly higher Om adipose tissue 20-HSD and 3-HSD-3 mRNA abundance compared to
women with low visceral adipose tissue accumulations (1.4 and 1.6 fold differences respectively,
p<0.05). Om and Sc adipose tissue 3-HSD activities were significantly higher in women with
high vs. low visceral adipose tissue areas (p<0.05 for both comparisons). Total and visceral
adiposity measures were positively associated with Om 20-HSD mRNA level (r=0.75, p<0.003
for fat mass and r=0.57, p<0.04 for visceral adipose tissue area) and Om 3-HSD-3 mRNA level
(r=0.68, p<0.01 for fat mass and r=0.74, p<0.003 for visceral adipose tissue area). Enzyme
activities in both the Sc and Om depots were also positively and significantly correlated with total
and abdominal adiposity measures, respectively. Omental adipose tissue enzyme expression and
activity were positively associated with omental adipocyte size and LPL activity. In conclusion,
mRNA abundance and activity of AKR1C enzymes in abdominal adipose tissue compartments
are positive correlates of adiposity measures in women. Further studies are required to elucidate
whether increased inactivation of progesterone and/or dihydrotestosterone in abdominal adipose
tissue impacts locally on fat metabolism in abdominally obese women.
This article has been cited by other articles:
|
|
 |
|
  C. Roberge, A. C. Carpentier, M.-F. Langlois, J.-P. Baillargeon, J.-L. Ardilouze, P. Maheux, and N. Gallo-Payet Adrenocortical dysregulation as a major player in insulin resistance and onset of obesity Am J Physiol Endocrinol Metab, December 1, 2007; 293(6): E1465 - E1478. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Blouin, C. Richard, G. Brochu, F.-S. Hould, S. Lebel, S. Marceau, S. Biron, V. Luu-The, and A. Tchernof Androgen inactivation and steroid-converting enzyme expression in abdominal adipose tissue in men J. Endocrinol., December 1, 2006; 191(3): 637 - 649. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K A Brown, D Boerboom, N Bouchard, M Dore, J G Lussier, and J Sirois Human chorionic gonadotropin-dependent induction of an equine aldo-keto reductase (AKR1C23) with 20{alpha}-hydroxysteroid dehydrogenase activity during follicular luteinization in vivo. J. Mol. Endocrinol., June 1, 2006; 36(3): 449 - 461. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
