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1 Copenhagen Muscle Research Centre, University of Copenhagen, August Krogh Institute, Copenhagen, Denmark
* To whom correspondence should be addressed. E-mail: cjuel{at}aki.ku.dk.
The study investigated the effect of training on lactate and H+ release from human skeletal muscle during one-legged knee-extensor exercise. Six subjects were tested after 7-8 weeks of training (fifteen 1-min bouts at ~150 % of thigh VO2max per day). Blood samples, blood flow, and muscle biopsies were obtained during and after a 30-W exercise bout and an incremental test to exhaustion of both trained (T-leg) and untrained (UT-leg) leg. Peak blood-flow was 16 % higher in T-leg than in UT-leg. In the 30-W test venous lactate and lactate release were lower in T-leg compared to UT-leg. In the incremental test time to fatigue was 10.6±0.7 and 8.2±0.7 min in T-leg and UT-leg (P<0.05). At exhaustion venous blood lactate was 10.7±0.4 and 8.0±0.9 mmol l-1 in T-leg and UT-leg (P<0.05), and lactate release was 19.4±3.6 and 10.6±2.0 mmol min-1 (P<0.05). H+ release at exhaustion was higher in T-leg than in UT-leg. Muscle lactate content was 59.0±15.1 and 96.5 ± 14.5 mmol kg-1 dry weight in T-leg and UT-leg, and muscle pH was 6.82±0.05 and 6.69±0.04 in T-leg and UT-leg (P = 0.06). The membrane contents of the monocarboxylate transporters MCT1 and MCT4 and the Na+/H+ exchanger were 115±5 (P<0.05), 111±11 and 116±6 % (P<0.05), respectively, in T-leg compared to UT-leg. The reason for the training-induced increase in peak lactate and H+ release during exercise is a combination of an increased density of the lactate and H+ transporting systems, an improved blood flow and blood flow distribution, as well as an increased systemic lactate and H+ clearance.
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