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1 Functional Muscle Genomics, AgResearch Ltd., Ruakura Agricultural Centre, Hamilton, New Zealand
2 Functional Muscle Genomics, AgResearch Ltd., Ruakura Agricultural Centre, Hamilton, New Zealand; Department of Pathology, University of Auckland, Auckland, New Zealand
3 Department of Sport and Exercise Science, University of Auckland, Auckland, New Zealand
4 Department of Pathology, University of Auckland, Auckland, New Zealand
* To whom correspondence should be addressed. E-mail: chris.mcmahon{at}agresearch.co.nz.
Myostatin inhibits myogenesis. Therefore, we sought to determine if mice lacking the myostatin gene (Mstn-/-) would lose less muscle mass than wild-type mice during seven days of hind limb suspension (HS). Male Mstn-/- and wild-type mice (C57) were subjected to HS or served as ground-based controls (n=6 per group). Wild-type mice lost 8% of body mass and ~13% of wet mass from biceps femoris, quadriceps femoris and soleus, while the mass of extensor digitorum longus (EDL) was unchanged after HS. Unexpectedly, Mstn-/- mice lost more body (13%, P<0.05) and quadriceps femoris mass (17%, P<0.05) than wild-type mice, and 33% of EDL mass (P<0.01) after HS. Protein expression of myostatin in biceps femoris and quadriceps femoris was not altered, whereas expression of MyoD, Myf-5 and myogenin increased in wild-type mice and tended to decrease of muscles of Mstn-/- mice. These data suggest that HS induced myogenesis in wild-type mice to counter atrophy, while myogenesis was not induced in Mstn-/- mice, thereby resulting in a greater loss of muscle mass.
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