AJP - Endo AJP: Lung Cellular and Molecular Physiology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
QUICK SEARCH:   [advanced]


     

Am J Physiol Endocrinol Metab (September 14, 2004). doi:10.1152/ajpendo.00256.2004
This Article
Right arrow Full Text (PDF)
Right arrow All Versions of this Article:
288/1/E187    most recent
00256.2004v1
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Baar, R. A.
Right arrow Articles by Parks, E. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Baar, R. A.
Right arrow Articles by Parks, E. J.
Submitted on June 15, 2004
Accepted on September 3, 2004

Investigation of in vivo fatty acid metabolism in AFABP/aP2-/- mice

Rachel A. Baar1, Carlus S. Dingfelder1, Lisa A. Smith2, Dave A. Bernlohr2, Chaodong Wu2, Alex J. Lange2, and Elizabeth J. Parks1*

1 Department of Food Science and Nutrition, University of Minnesota, St. Paul, MN, USA
2 Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN, USA

* To whom correspondence should be addressed. E-mail: eparks{at}umn.edu.

The metabolic impact of the murine adipocyte fatty acid binding protein (AFABP/aP2) on lipid metabolism was investigated in the AFABP/aP2-/- mouse and compared to wild type C57Bl/6J littermates. Mice weaned on a high-fat diet (59% of energy from fat) and acclimated to meal feeding. Stable isotopes were administered and indirect calorimetry performed to quantitate fatty acid flux, dietary fatty acid utilization, and substrate oxidation. Consistent with previous in situ and in vitro studies, fasting serum non-esterified fatty acids (NEFA) release was significantly reduced in AFABP/aP2-/- (17.1 ± 9.0 vs 51.9 ± 22.9 mg/kg/min). AFABP/aP2-/- exhibited higher serum NEFA (1.4 ± 0.6 vs 0.8 ± 0.4 mmol/L, AFABP/aP2-/- vs C57Bl/6J, respectively), triacylglycerol (TAG) (0.23 ± 0.09 vs 0.13 ± 0.10 mmol/L), and accumulated more TAG in liver tissue (2.9 ± 2.3 vs 1.1 ± 0.8 % wet weight) in the fasted state. For the liver-TAG pool, 16.4 ± 7.3% of TAG-fatty acids were derived from serum NEFA in AFABP/aP2-/-. In contrast, a significantly greater portion of C57Bl/6J liver-TAG was derived from serum NEFA (42.3 ± 25.5%) during tracer infusion. For adipose-TAG stores, only 0.29 ± 0.04% was derived from serum NEFA in AFABP/aP2-/-, and in C57Bl/6J, 1.85 ± 0.97% of adipose-TAG was derived from NEFA. In addition, AFABP/aP2-/- preferentially oxidized glucose relative to fatty acids in the fed state. These data demonstrate in vivo disruption of AFABP/aP2-/- leads to changes in two major metabolic processes; 1) decreased adipose NEFA efflux and 2) preferential utilization of glucose relative to fatty acids.




This article has been cited by other articles:


Home page
 J EndocrinolHome page
K.-A. Kim, S.-O Ka, W. S. Moon, H.-K. Yi, Y.-H. Lee, K.-B. Kwon, J.-W. Park, and B.-H. Park
Effect of dermcidin, an antimicrobial peptide, on body fat mobilization in normal mice
J. Endocrinol., July 1, 2008; 198(1): 111 - 118.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
A. V. Hertzel, L. A. Smith, A. H. Berg, G. W. Cline, G. I. Shulman, P. E. Scherer, and D. A. Bernlohr
Lipid metabolism and adipokine levels in fatty acid-binding protein null and transgenic mice
Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E814 - E823.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Chem.Home page
A. Xu, Y. Wang, J. Y. Xu, D. Stejskal, S. Tam, J. Zhang, N. M.S. Wat, W. K. Wong, and K. S.L. Lam
Adipocyte Fatty Acid-Binding Protein Is a Plasma Biomarker Closely Associated with Obesity and Metabolic Syndrome
Clin. Chem., March 1, 2006; 52(3): 405 - 413.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH
Visit Other APS Journals Online
Copyright © 2004 by the American Physiological Society.