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Am J Physiol Endocrinol Metab (July 12, 2005). doi:10.1152/ajpendo.00237.2005
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Submitted on May 27, 2005
Accepted on July 7, 2005

Endurance Training Increases Skeletal Muscle LKB1 and PGC-1{alpha} Protein Abundance: Effects of Time and Intensity

Eric B Taylor1, Jeremy D Lamb1, Richard W Hurst1, David G Chesser1, William J Ellingson1, Lyle J Greenwood1, Brian B Porter1, Seth T Herway1, and William W Winder1*

1 Department of Physiology and Developmental Biology, Brigham Young University, Provo, UT, USA

* To whom correspondence should be addressed. E-mail: william_winder{at}byu.edu.

Recent research suggests LKB1 is the major AMP-activated protein kinase (AMPK) kinase (AMPKK). Peroxisome-proliferator-activated receptor-{gamma} coactivator 1{alpha} (PGC-1{alpha}) is a master coordinator of mitochondrial biogenesis. Previously we reported skeletal muscle LKB1 protein increases with endurance training. The purpose of this study was to determine if training induced increases in skeletal muscle LKB1 and PGC-1{alpha} protein exhibit a time-course and intensity dependent response similar to citrate synthase. Male Sprague-Dawley rats completed endurance and interval training protocols. For endurance training, rats trained for 4, 11, 25, or 53 days. Interval training rats trained identically to endurance trained rats except that after 25 days, interval training was combined with endurance training. Time-course data was collected from endurance trained red quadriceps (RQ) after each time point. Interval training data was collected from soleus, RQ, and white quadriceps (WQ) muscle after 53 days only. MO25 and PGC-1{alpha} protein increased significantly after 4 days. Increased citrate synthase activity, increased LKB1 protein, and decreased AMPKK activity were found after 11 days. Maximal increases occurred after 4 days for hexokinase-2, 25 days for MO25, and 53 days for citrate synthase, LKB1, and PGC-1{alpha}. In WQ, but not RQ or soleus, interval training had an additive effect to endurance training and induced significant increases in all proteins measured. These results demonstrate that LKB1 and PGC-1{alpha} protein abundances increase with endurance and interval training similarly to citrate synthase. The increase in LKB1 and PGC-1{alpha} with endurance and interval training may function to maintain the training induced increases in mitochondrial mass.




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