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Articles in PresS, published online ahead of print January 22, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00215.2001
Submitted on May 16, 2001
Accepted on January 15, 2002
1 Department of Surgery, State University of New York, Stony Brook, NY, USA
2 Endocrine Research Unit, Division of Endocrinology and Metabolism, Mayo Foundation, Rochester, MN, USA
* To whom correspondence should be addressed. E-mail: Giuseppe.Caso{at}stonybrook.edu.
Muscle protein synthesis measured by flooding with L-[2H5]phenylalanine (70 mg/kg) was significantly stimulated by infusion of insulin with amino acids in dogs. The stimulation of muscle protein synthesis was similar when calculated from the enrichment of phenylalanyl-tRNA (61±10%, P<0.001), plasma phenylalanine (61±10%, P<0.001) or tissue fluid phenylalanine (54±10%, P<0.001). The time course for changes in enrichment of L-[2H5]phenylalanine throughout the flooding period was determined for plasma, tissue fluid, and phenylalanyl-tRNA, in the basal state and during the infusion of insulin with amino acids. Enrichments of plasma free phenylalanine and phenyalanyl-tRNA were equalized between 20 and 45 min, although the enrichment of phenyalanyl-tRNA was lower at early time points. Rates of muscle protein synthesis obtained with the flooding method and calculated from plasma phenylalanine enrichment were comparable to those calculated from phenyalanyl-tRNA and also to those obtained previously with a continuous infusion of phenylalanine with phenyalanyl-tRNA as precursor. This study confirms that with a bolus injection of labeled phenylalanine the enrichment of aminoacyl-tRNA, the true precursor pool for protein synthesis, can be accurately assessed from more readily sampled plasma phenylalanine.
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