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Am J Physiol Endocrinol Metab (November 26, 2002). doi:10.1152/ajpendo.00213.2002
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Articles in PresS, published online ahead of print November 26, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00213.2002
Submitted on May 14, 2002
Accepted on November 17, 2002

The Influence of Growth Hormone on Bone Marrow Adipogenesis in Hypophysectomized Rats

Yaw Appiagyei-Dankah1*, Carmen D. Tapiador1, Jodi F. Evans2, Mariano Castro-Magana3, John F. Aloia4, and James K. Yeh4

1 Division of Pediatric Endocrinology, Winthrop-University Hospital, Mineola, NY, USA
2 Department of Medicine, Winthrop-University Hospital, Mineola, NY, USA
3 Division of Pediatric Endocrinology, Winthrop-University Hospital, Mineola, NY, USA; The Health Sciences Center, State University of New York, Stony Brook, Stony Brook, NY, USA
4 Department of Medicine, Winthrop-University Hospital, Mineola, NY, USA; The Health Sciences Center, State University of New York, Stony Brook, Stony Brook, NY, USA

* To whom correspondence should be addressed. E-mail: yappiagyei{at}yahoo.com.

The hypophysectomized rat has been used as a model to study the effects of growth hormone deficiency on bone. Here we have investigated the influence of growth hormone administration to hypophysectomized rats for 6 weeks on accumulation of triglycerides in bone marrow and, on the differentiation of primary marrow stromal cells into adipocytes under in vitro conditions. We found that hypophysectomy significantly increased triglyceride concentration in bone marrow which was attenuated by growth hormone administration. Primary bone marrow stromal cells derived from hypophysectomized rats (HX) also had more adipocytes at confluence compared to growth hormone treated rats (GH). When stimulated with IBMX/Dex, preadipocyte colony counts increased more significantly in GH. Markers of adipocyte differentiation were higher in HX than CT or GH at confluence. However, following stimulation with IBMX/Dex, increased expression of markers was seen in GH compared to HX. Conclusion: Growth hormone administration to hypophysectomized rats attenuated triglyceride accumulation in bone marrow, and inhibited the differentiation of stromal cells into adipocytes in vitro.




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