| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Medicine, Boston University, Boston, Massachusetts, United States
2 Genetics and Genomics, Boston University, Boston, Massachusetts, United States
3 Medicine, Beth Israel Deaconess Medical Center, Boston, Massachusetts, United States
4 Surgery, Creighton University, Omaha, Nebraska, United States
5 Endocrine Research Unit, Mayo Clinic Foundation, Rochester, Minnesota, United States
6 Endocrine Research Unit, Mayo Clinic, Rochester, Minnesota, United States
7 Medicine, Boston University Medical Center, Boston, Massachusetts, United States
* To whom correspondence should be addressed. E-mail: kirkland{at}bu.edu.
Anatomically separate fat depots differ in size, function, and contribution to pathological states such as the metabolic syndrome. We isolated preadipocytes from different human fat depots to determine if the basis for this variation is partly attributable to differences in inherent properties of fat cell progenitors. We found that genome-wide expression profiles of primary preadipocytes cultured in parallel from abdominal subcutaneous, mesenteric, and omental fat depots were distinct. Interestingly, visceral fat was not homogeneous. Preadipocytes from one of the two main visceral depots, mesenteric fat, had an expression profile closer to that of subcutaneous than omental preadipocytes, the other main visceral depot. Expression of genes that regulate early development, including homeotic genes, differed extensively among undifferentiated preadipocytes isolated from different fat depots. These profiles were confirmed by real time PCR analysis of preadipocytes from additional lean and obese, male and female subjects. We made preadipocyte strains from single abdominal subcutaneous and omental preadipocytes by expressing telomerase. Depot-specific developmental gene expression profiles persisted for 40 population doublings in these strains. Thus, human fat cell progenitors from different regions are effectively distinct, consistent with different fat depots being separate mini-organs.
This article has been cited by other articles:
|
|
 |
|
  F. Giorgino Adipose tissue function and dysfunction: organ cross talk and metabolic risk Am J Physiol Endocrinol Metab, November 1, 2009; 297(5): E975 - E976. [Full Text] [PDF]
|
|
|
|
 |
|
 S. Baglioni, M. Francalanci, R. Squecco, A. Lombardi, G. Cantini, R. Angeli, S. Gelmini, D. Guasti, S. Benvenuti, F. Annunziato, et al. Characterization of human adult stem-cell populations isolated from visceral and subcutaneous adipose tissue FASEB J, October 1, 2009; 23(10): 3494 - 3505. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gross, I. Karagiannides, T. Thomou, H. W. Koon, C. Bowe, H. Kim, N. Giorgadze, T. Tchkonia, T. Pirtskhalava, J. L. Kirkland, et al. Substance P promotes expansion of human mesenteric preadipocytes through proliferative and antiapoptotic pathways Am J Physiol Gastrointest Liver Physiol, May 1, 2009; 296(5): G1012 - G1019. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. J. Hausman, M. V. Dodson, K. Ajuwon, M. Azain, K. M. Barnes, L. L. Guan, Z. Jiang, S. P. Poulos, R. D. Sainz, S. Smith, et al. BOARD-INVITED REVIEW: The biology and regulation of preadipocytes and adipocytes in meat animals J Anim Sci, April 1, 2009; 87(4): 1218 - 1246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. K. Chatterjee, L. L. Stoll, G. M. Denning, A. Harrelson, A. L. Blomkalns, G. Idelman, F. G. Rothenberg, B. Neltner, S. A. Romig-Martin, E. W. Dickson, et al. Proinflammatory Phenotype of Perivascular Adipocytes: Influence of High-Fat Feeding Circ. Res., February 27, 2009; 104(4): 541 - 549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Blouin, M. Nadeau, J. Mailloux, M. Daris, S. Lebel, V. Luu-The, and A. Tchernof Pathways of adipose tissue androgen metabolism in women: depot differences and modulation by adipogenesis Am J Physiol Endocrinol Metab, February 1, 2009; 296(2): E244 - E255. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Suzuki, S. Sembon, M. Iwamoto, D. Fuchimoto, and A. Onishi Identification of genes downregulated during differentiation of porcine mesenteric adipocytes J Anim Sci, December 1, 2008; 86(12): 3367 - 3376. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Pantoja, J. T. Huff, and K. R. Yamamoto Glucocorticoid Signaling Defines a Novel Commitment State during Adipogenesis In Vitro Mol. Biol. Cell, October 1, 2008; 19(10): 4032 - 4041. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Gondret, N. Guitton, C. Guillerm-Regost, and I. Louveau Regional differences in porcine adipocytes isolated from skeletal muscle and adipose tissues as identified by a proteomic approach J Anim Sci, September 1, 2008; 86(9): 2115 - 2125. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Y. Kim, Y. Wu, and C. M. Smas Characterization of ScAP-23, a new cell line from murine subcutaneous adipose tissue, identifies genes for the molecular definition of preadipocytes Physiol Genomics, October 19, 2007; 31(2): 328 - 342. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
