| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
|
|
|
|||||||
|
|||||||||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
1 Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah, USA
* To whom correspondence should be addressed. E-mail: william_winder{at}byu.edu.
LKB1 complexed with MO25 and STRAD has been identified as an AMP-activated
protein kinase (AMPK) kinase (AMPKK). We measured relative LKB1 protein
abundance and AMPKK activity in liver (LV), heart (HT), soleus (SO), red quadriceps
(RQ), and white quadriceps (WQ) from sedentary and endurance trained rats. We
examined trained RQ for altered levels of MO25 protein and LKB1, STRAD, and MO25
mRNA. LKB1 protein levels normalized to HT (1 ± 0.03) were LV (0.50 ± 0.03), SO
(0.28 ± 0.02), RQ (0.32 ± 0.01), and WQ (0.12 ± 0.03). AMPKK activities in
nmoles/g/min were HT (79 ± 6), LV (220 ± 9), SO (22 ± 2), RQ (29 ± 2), and WQ (42 ±
4). Training increased LKB1 protein in SO, RQ, and WQ (p < 0.05). LKB1 protein levels
after training (% controls) were SO (158 ± 17), RQ (316 ± 17), WQ (191 ± 27), HT (106
± 2), and LV (104 ± 7). MO25 protein (% controls) after training was 595 ± 71. Training
did not affect AMPKK activity. MO25 but not LKB1 or STRAD mRNA increased with
training (p < 0.05). Trained values (% controls) were MO25 (164 ± 22), LKB1 (120 ±
16), and STRAD (112 ± 17). LKB1 protein content strongly correlated (r = 0.93) with
citrate synthase activity in skeletal muscle (p < 0.05). In conclusion, endurance training
markedly increased skeletal muscle LKB1 and MO25
protein without increasing
AMPKK activity. LKB1 may be playing multiple roles in skeletal muscle adaptation to
endurance training.
This article has been cited by other articles:
|
|
 |
|
  D. J. Branvold, D. R. Allred, D. J. Beckstead, H. J. Kim, N. Fillmore, B. M. Condon, J. D. Brown, S. N. Sudweeks, D. M. Thomson, and W. W. Winder Thyroid hormone effects on LKB1, MO25, phospho-AMPK, phospho-CREB, and PGC-1{alpha} in rat muscle J Appl Physiol, October 1, 2008; 105(4): 1218 - 1227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. J. Ellingson, D. G. Chesser, and W. W. Winder Effects of 3-phosphoglycerate and other metabolites on the activation of AMP-activated protein kinase by LKB1-STRAD-MO25 Am J Physiol Endocrinol Metab, February 1, 2007; 292(2): E400 - E407. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. B. Birk and J. F. P. Wojtaszewski Predominant {alpha}2/{beta}2/{gamma}3 AMPK activation during exercise in human skeletal muscle J. Physiol., December 15, 2006; 577(3): 1021 - 1032. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Xue and B. B. Kahn AMPK integrates nutrient and hormonal signals to regulate food intake and energy balance through effects in the hypothalamus and peripheral tissues J. Physiol., July 1, 2006; 574(1): 73 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Sriwijitkamol, J. L. Ivy, C. Christ-Roberts, R. A. DeFronzo, L. J. Mandarino, and N. Musi LKB1-AMPK signaling in muscle from obese insulin-resistant Zucker rats and effects of training Am J Physiol Endocrinol Metab, May 1, 2006; 290(5): E925 - E932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Taylor, W. J. Ellingson, J. D. Lamb, D. G. Chesser, C. L. Compton, and W. W. Winder Evidence against regulation of AMP-activated protein kinase and LKB1/STRAD/MO25 activity by creatine phosphate Am J Physiol Endocrinol Metab, April 1, 2006; 290(4): E661 - E669. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Taylor, J. D. Lamb, R. W. Hurst, D. G. Chesser, W. J. Ellingson, L. J. Greenwood, B. B. Porter, S. T. Herway, and W. W. Winder Endurance training increases skeletal muscle LKB1 and PGC-1{alpha} protein abundance: effects of time and intensity Am J Physiol Endocrinol Metab, December 1, 2005; 289(6): E960 - E968. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Hurst, E. B. Taylor, T. D. Cline, L. J. Greenwood, C. L. Compton, J. D. Lamb, and W. W. Winder AMP-activated protein kinase kinase activity and phosphorylation of AMP-activated protein kinase in contracting muscle of sedentary and endurance-trained rats Am J Physiol Endocrinol Metab, October 1, 2005; 289(4): E710 - E715. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Jessen and L. J. Goodyear Contraction signaling to glucose transport in skeletal muscle J Appl Physiol, July 1, 2005; 99(1): 330 - 337. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. B. Taylor, W. J. Ellingson, J. D. Lamb, D. G. Chesser, and W. W. Winder Long-chain acyl-CoA esters inhibit phosphorylation of AMP-activated protein kinase at threonine-172 by LKB1/STRAD/MO25 Am J Physiol Endocrinol Metab, June 1, 2005; 288(6): E1055 - E1061. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH |
| Visit Other APS Journals Online |
