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1 Department of Physiology and Developmental Biology, Brigham Young University, Provo, Utah, USA
* To whom correspondence should be addressed. E-mail: william_winder{at}byu.edu.
LKB1 complexed with MO25 and STRAD has been identified as an AMP-activated
protein kinase (AMPK) kinase (AMPKK). We measured relative LKB1 protein
abundance and AMPKK activity in liver (LV), heart (HT), soleus (SO), red quadriceps
(RQ), and white quadriceps (WQ) from sedentary and endurance trained rats. We
examined trained RQ for altered levels of MO25 protein and LKB1, STRAD, and MO25
mRNA. LKB1 protein levels normalized to HT (1 ± 0.03) were LV (0.50 ± 0.03), SO
(0.28 ± 0.02), RQ (0.32 ± 0.01), and WQ (0.12 ± 0.03). AMPKK activities in
nmoles/g/min were HT (79 ± 6), LV (220 ± 9), SO (22 ± 2), RQ (29 ± 2), and WQ (42 ±
4). Training increased LKB1 protein in SO, RQ, and WQ (p < 0.05). LKB1 protein levels
after training (% controls) were SO (158 ± 17), RQ (316 ± 17), WQ (191 ± 27), HT (106
± 2), and LV (104 ± 7). MO25 protein (% controls) after training was 595 ± 71. Training
did not affect AMPKK activity. MO25 but not LKB1 or STRAD mRNA increased with
training (p < 0.05). Trained values (% controls) were MO25 (164 ± 22), LKB1 (120 ±
16), and STRAD (112 ± 17). LKB1 protein content strongly correlated (r = 0.93) with
citrate synthase activity in skeletal muscle (p < 0.05). In conclusion, endurance training
markedly increased skeletal muscle LKB1 and MO25
protein without increasing
AMPKK activity. LKB1 may be playing multiple roles in skeletal muscle adaptation to
endurance training.
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