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Am J Physiol Endocrinol Metab (November 26, 2002). doi:10.1152/ajpendo.00153.2002
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Articles in PresS, published online ahead of print November 26, 2002
Am J Physiol Endocrinol Metab, 10.1152/ajpendo.00153.2002
Submitted on April 10, 2002
Accepted on November 21, 2002

Non-nutritive flow impairs uptake of fatty acid by white muscles of the perfused rat hindlimb

Lucy H. Clerk1, Maree E. Smith1, Stephen Rattigan1, and Michael G. Clark1*

1 Department of Biochemistry, University of Tasmania, Hobart, Tasmania, Australia

* To whom correspondence should be addressed. E-mail: Michael.Clark{at}utas.edu.au.

Triglyceride hydrolysis by the perfused rat hindlimb is enhanced with serotonin-induced non-nutritive flow (NNF) and may be due to the presence of non-nutritive route-associated connective tissue fat cells. Here we assess whether NNF influences muscle uptake of 0.55mM palmitate in the perfused hindlimb. Comparisons were made with insulin-mediated glucose uptake. NNF induced during 60nM insulin infusion inhibited hindlimb oxygen uptake from 22.0±0.5 to 9.7±0.8 µmol/g/h (P<0.001), 1-methyl xanthine metabolism (indicator of nutritive flow) from 5.8±0.4 to 3.8±0.4 nmol/min/g (P=0.004), glucose uptake from 29.2±1.7 to 23.1±1.8 µmol/g/h (P=0.005) and muscle 2-deoxyglucose uptake from 82.1±4.6 to 41.6±6.7 µmol/g/h (P<0.001). Palmitate uptake, unaffected by insulin alone, was inhibited by NNF in EDL, gastrocnemius white, and tibialis muscles; average inhibition was from 13.9±1.2 to 6.9±1.4 µmol/g/h (P=0.02). Thus NNF impairs both fatty acid and glucose uptake by muscle by restricting flow to myocytes but, as shown previously, favors triglyceride hydrolysis and uptake into nearby connective tissue fat cells. The findings have implications for lipid partitioning in limb muscles between myocytes and attendant adipocytes.




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