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1 Department of Nutrition, Case Western Reserve University, Cleveland, OH, USA
2 Department of Mathematics, Case Western Reserve University, Cleveland, OH, USA
3 Department of Medicine, Case Western Reserve University, Cleveland, OH, USA
* To whom correspondence should be addressed. E-mail: sxp29{at}po.cwru.edu.
We have studied the accretion of lipids in growing mice. We measured the rates of synthesis and degradation of triglycerides in epididymal fat pads of mice maintained for 44 days on a low-fat, high-carbohydrate diet (I) or a high-fat, low-carbohydrate diet (II). 2H2O was added to the drinking water for 14 days. Rates of incorporation/washout of 2H to/from carbon 1 of triglyceride-glycerol showed that triglyceride synthesis was greater than triglyceride degradation (the net triglyceride balance was ~ 2.5 times greater in II vs I). The data also show that the contribution of de novo lipogenesis to triglyceride-bound palmitate was ~ 3 times greater in I vs II. This was consistent with a greater relative intake of carbohydrate in I vs II. The rates of incorporation and washout of newly synthesized (i.e. 2H-labeled) palmitate into, and from, triglycerides were also measured. Those data suggested a remodeling of triglyceride-bound fatty acids. On measuring the profile of triglyceride-bound fatty acids we observed a decrease in the relative abundance of triglyceride-bound palmitate and stearate and an increase in triglyceride-bound oleate and linoleate. This was observed in I and II. In summary, diet substantially affects the deposition and the modeling of triglycerides in adipose tissue during growth. 2H2O can be used to examine the mechanisms responsible for the accumulation of triglycerides, e.g. factors that affect (i) triglyceride synthesis and degradation and (ii) the source of fatty acids that are used in esterification.
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